Table 8 Comparison between existing and proposed method

Solvent

Acetonitrile (more expensive)

Methanol (less expensive)

Mobile phase mixture

Acetonitrile-ammonium acetate

Methanol–water

Retention time

7.20 ± 0.07 min(longer)

5.09 ± 0.02 min (shorter)

LOD

9.5 ng/mL

0.35 μg/mL

LOQ

30.6 ng/mL

1.08 μg/mL

Peak tailing factor

1.5 (good)

1.32 (better)