Table 1 Optimized chromatographic conditions
Instruments | WATER UPLC Acquit model with auto sampler and PDA detector |
|---|---|
Temperature | 25 °C |
Mode of separation | Isocratic mode |
Column | Dismal leapsil C 18 column (4.6 × 50 mm, 2.7 µm) |
Mobile phase | 0.1% orthophosphoric acid: methanol (40:60) |
Flow rate | 0.3 ml/min |
Wavelength | 224 nm |
Injection volume | 4 Micron liter |
Run time | 6 min |