Table 3 Recovery and matrix effect of CXB and IS in rat plasma (n = 6)
Statistical variable | Nominal CXB concentration (ng/mL) | Nominal IS concentration (ng/mL) | |||
|---|---|---|---|---|---|
CXB matrix effect | 10 | 400 | 800 | 1000 | 400 |
Mean ± SD (%) | 94.12 ± 2.80 | 96.23 ± 2.56 | 95.83 ± 1.90 | 97.15 ± 3.50 | 95.97 ± 2.45 |
RSD (%) | 2.97 | 2.66 | 5.04 | 3.60 | 2.25 |
CXB recovery | 10 | 400 | 800 | 1000 | 400 |
Mean ± SD (%) | 8.640 ± 0.55 | 387.3 ± 5.85 | 790.5 ± 6.53 | 948.9 ± 12.04 | 386.4 ± 9.37 |
RSD (%) | 8.90 | 1.51 | 2.71 | 1.27 | 4.57 |
ACF matrix effect | 100 | 450 | 900 | 200 | |
Mean ± SD (%) | 94.03 ± 5.63 | 92.78 ± 3.22 | 90.25 ± 1.47 | 91.38 ± 2.63 | |
RSD (%) | 5.98 | 3.47 | 1.62 | 2.87 | |
ACF recovery | 100 | 450 | 900 | 200 | |
Mean ± SD (%) | 95.74 ± 2.45 | 444.4 ± 4.00 | 884.8 ± 3.19 | 195.5 ± 2.57 | |
RSD (%) | 2.56% | 0.90% | 0.36% | 1.32% | |