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Fig. 4 | Future Journal of Pharmaceutical Sciences

Fig. 4

From: Pharmacognostic standardisation of the leaves of Costus afer Ker Gawl. (Zingiberaceae) and Palisota hirsuta (Thunb.) K. Schum. (Commelinaceae)

Fig. 4

Transverse sections of mid rib of leaves × 100; A Costus afer B Palisota hirsute. CO: collenchyma; CU: Cuticle; LE PP: palisade parenchyma; UE: upper epidermis, VB: vascular bundle. Briefly, scalpel was used to cut a tiny strip of the plants through the midrib region. The transverse sections obtained were cleared using 2% v/v sodium hypochlorite, washed severally in water, stained with Safranin O, dehydrated in 50%, 70% and 100% Ethanol, mounted in dilute, glycerol, viewed under microscope and, photomicrograph taken, with the, aid, of phone camera

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