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Table 3 Highly expressed eggshell membrane protein showing sequences with A-Cell epitopes and their physicochemical properties

From: Could eggshell membrane be an adjuvant for recombinant Hepatitis B vaccine?: A preliminary investigation

 

NCBI accession no

Protein

LC–MS/MS Scores by Rath et al. [20]

If A-cell Epitopes Present ( +) or Absent (−)

Immuno-modulatory sequence coverage

pI

Peptide MW (kDa)

GRAVY

Aliphatic index

1

QBB01285.1

Ovalbumin

629.9 (11)

 +  +  +  + 

FRADHPFLFCIKHIATNAVLFFGRCVSP

8.96

3.207

0.643

87.14

2

P01005.1

Ovomucoid

338.1 (7)

−

–

–

–

–

–

3

XP_015131596.1

Ovocleidin-116

225 (2)

−

–

–

–

–

–

4

NP_989872.1

Orosomucoid 1

167 (3)

−

–

–

–

–

–

5

XP_015151807.1

Peptidyl-prolyl cis–trans isomerase

163.5 (3)

−

–

–

–

–

–

6

NP_990612.1

Lysozyme C

129.2 (2)

 +  +  +  + 

ASVNCAKKIVSDGNGMSAWVAWRNRCKG

9.85

3.009

−0.318

59.29

7

XP_015128103.2

Keratin 8, Type II

119.1 (2)

−

–

–

–

–

–

8

NP_001012592.1

SH3 domain binding (SH3BGRL)

109.6 (2)

−

–

–

–

–

–

9

NP_001025998.1

Ubiquitin-fold modifier 1

102.1 (2)

 +  +  + 

PAQTAGNVFLKHGSDLRIIPRDRVGSS

10.74

2892.27

0.396

86.67

10

Q6QLR1.1

Gallinacin-9

85.7 (1)

−

–

–

–

–

–

  1. The number parenthesis is the number of peptides obtain in the LC–MS/MS analysis as conducted by Rath et al. [13]. The number of ‘ + ’ gives an indication of number of A-cell epitope in each IM protein as predicted by VaxinPad