HPLC fingerprint of flavonoids, enzyme inhibition and antioxidant activity of Newbouldia laevis stem-bark: an in vitro and in silico study

Future Journal of Pharmaceutical Sciences

Table 3 Enzyme inhibitions potentials of Newbouldia laevis stem-bark extract

Enzyme inhibition	100 mg/mL	200 mg/mL	300 mg/mL	500 mg/mL	Standard inhibitors (mg/mL)
Acetylcholinesterase (%)	58.12^bA ± 0.15	63.62^bA ± 1.04	52.59^cA ± 1.08	62.17^dA ± 0.27	89.76^a ± 2.87 (Galantamine)
Butyrylcholinesterase (%)	60.38^bB ± 0.90	71.08^bB ± 0.18	64.30^cB ± 0.32	89.71^dB ± 1.93	91.59^a ± 1.18 (Galantamine)
Phospholipase A₂ (%)	87.77^bA ± 0.66	91.83^bC ± 1.60	96.15^cB ± 1.93	84.53^dB ± 1.44	94.76^a ± 1.00 (Prednisolone)
α-Glucosidase (%)	65.20^bC ± 0.43	65.19^bD ± 0.51	69.84^cB ± 0.33	71.21^cC ± 0.09	87.51^a ± 0.39 (Acarbose)
α-Amylase	55.66^bC ± 1.17	59.85^bC ± 2.13	66.82^bC ± 1.04	60.82^bC ± 0.39	79.52^a ± 0.51 (Acarbose)

Values were of triplicate tests, (mean ± standard error (SE); values with different lowercase are statistically significant (p < 0.05) compared to Standard inhibitors; values with different uppercase are statistically significant (p < 0.05) across concentrations