Table 4 Robustness results of the proposed method

Flow rate (mL/min)

0.9

3.95

1.09

0.35

1.1

2.40

1.39

0.21

Wavelength (nm)

208 nm

3.08

1.12

0.19

212 nm

3.09

1.13

0.42

Column temperature (°C)

27

3.21

1.09

1.04

33

2.94

1.18

0.49

Acetonitrile ratio

48.5%

3.47

1.14

0.58

51.5%

2.89

1.23

0.79

Acceptance value

–

0.8–2.0

< 2