The phytochemical screening, total cucurbitacin content, and in vitro anti-breast cancer activity of Leucopaxillus gentianeus mushroom

Future Journal of Pharmaceutical Sciences

Table 11 HPTLC-Cucurbitacin profile of the standard sample

Amount	Peak	Start Rf	Start Height	Max Rf	Max Height	Max %	End Rf	End Height	Area	Area %	Assigned substance
40 ng	1	0.55	3.9	0.56	13.6	2.53	0.57	1.6	97.7	0.32	Unknown*
	2	0.83	0.5	0.89	523.9	97.47	0.98	9.1	30,386.9	99.68	Cucurbitacin
60 ng	1	0.81	0.3	0.89	552.7	100.0	0.98	2.5	29,701.9	100.00	Cucurbitacin
100 ng	1	0.76	5.3	0.89	612.7	100.0	0.98	1.5	32,354.5	100.00	Cucurbitacin
140 ng	1	0.79	23.9	0.89	656.7	89.87	0.95	65.6	34,849.4	96.22	Cucurbitacin
	2	0.95	65.6	0.96	74.0	10.13	0.98	4.7	1367.4	3.78	Unknown*
160 ng	1	0.13	1.8	0.17	16.7	2.30	0.20	12.8	761.6	1.93	Unknown*
	2	0.66	5.9	0.71	16.0	2.21	0.71	13.7	478.7	1.21	Unknown*
	3	0.78	29.1	0.89	693.5	95.49	0.98	3.3	38,313.2	96.86	Cucurbitacin
200 ng	1	0.75	15.0	0.89	700.4	100.0	0.98	0.9	39,450.0	100.00	Cucurbitacin
240 ng	1	0.75	11.2	0.89	689.5	100.0	0.98	2.8	39,929.5	100.0	Cucurbitacin