Table 1 The accuracy, precision and recovery for the assay of efavirenz
Theoretical concentration | Intra-day | Inter-day | ||||
|---|---|---|---|---|---|---|
Assay (mean ± SD) | Accuracy (%) | Precision (%) | Assay (mean ± SD) | Accuracy (%) | Precision (%) | |
Accuracy and precision for Efavirenz | ||||||
Low (500 ng/mL) | 585.85 ± 76.89 | 117.17 | 13.12 | 578.85 ± 7.35 | 115.77 | 1.27 |
Medium (4500 ng/mL) | 4685.12 ± 72.21 | 104.11 | 1.54 | 4776.50 ± 83.07 | 106.14 | 1.74 |
High (18,000 ng/mL) | 20,024.70 ± 150.21 | 111.25 | 0.75 | 19,993.93 ± 85.30 | 111.08 | 0.43 |
Accuracy and precision for lumefantrine | ||||||
Low (400 ng/mL) | 412.36 ± 20.53 | 103.09 | 4.91 | 420.55 ± 10.01 | 105.14 | 2.38 |
Medium (2000 ng/mL) | 1907.28 ± 122.95 | 95.36 | 6.45 | 1913.30 ± 22.00 | 95.36 | 1.15 |
High (15,000 ng/mL) | 15,171.61 ± 678.18 | 101.14 | 4.47 | 14,702.94 ± 427.15 | 98.02 | 2.91 |
Recoveries for efavirenz and lumefantrine | |||
|---|---|---|---|
Efavirenz | Lumefantrine | ||
Theoretical concentration | Recovery (%) | Theoretical concentration | Recovery (%) |
Low (500 ng/mL) | 117.13 ± 2.28 | Low (400 ng/mL) | 75.27 ± 1.82 |
Medium (4500 ng/mL) | 109.08 ± 2.38 | Medium (2000 ng/mL) | 72.64 ± 0.84 |
High (18,000 ng/mL) | 111.72 ± 3.09 | High (15,000 ng/mL) | 76.62 ± 2.16 |