Table 2 Optimization of parameters using BBD design
Std. | Run | Factor 1 | Factor 2 | Factor 3 | Response 1 | Response 2 | Response 3 |
|---|---|---|---|---|---|---|---|
A: % Organic modifier (ml) | B: pH | C: Flow rate (ml/min) | Retention time (min) | Tailing factor | Theoretical plate | ||
9 | 1 | 60 | 4 | 0.8 | 4.128 | 1.312 | 2878 |
7 | 2 | 55 | 4.5 | 1.2 | 2.592 | 1.328 | 4328 |
8 | 3 | 65 | 4.5 | 1.2 | 2.198 | 1.273 | 4824 |
5 | 4 | 55 | 4.5 | 0.8 | 4.231 | 1.362 | 2549 |
1 | 5 | 55 | 4 | 1 | 4.135 | 1.324 | 2963 |
14 | 6 | 60 | 4.5 | 1 | 2.486 | 1.287 | 3384 |
10 | 7 | 60 | 5 | 0.8 | 3.821 | 1.351 | 2854 |
15 | 8 | 60 | 4.5 | 1 | 2.476 | 1.279 | 3174 |
4 | 9 | 65 | 5 | 1 | 3.125 | 1.298 | 3176 |
12 | 10 | 60 | 5 | 1.2 | 2.542 | 1.273 | 5137 |
6 | 11 | 65 | 4.5 | 0.8 | 3.211 | 1.338 | 2385 |
3 | 12 | 55 | 5 | 1 | 3.482 | 1.345 | 3512 |
13 | 13 | 60 | 4.5 | 1 | 2.468 | 1.284 | 3312 |
11 | 14 | 60 | 4 | 1.2 | 2.725 | 1.296 | 4825 |
2 | 15 | 65 | 4 | 1 | 3.084 | 1.307 | 3578 |