Table 3 ANOVA responses of BBD design
Source | Sum of squares | df | Mean square | F-value | p value | |
|---|---|---|---|---|---|---|
For retention time | ||||||
Model | 6.77 | 9 | 0.7523 | 1757.57 | < 0.0001 | Significant |
A—% Organic modifier | 0.9955 | 1 | 0.9955 | 2325.66 | < 0.0001 | |
B—pH | 0.1518 | 1 | 0.1518 | 354.65 | < 0.0001 | |
C—Flow rate | 3.56 | 1 | 3.56 | 8308.80 | < 0.0001 | |
For tailing factor | ||||||
Model | 0.0121 | 9 | 0.0013 | 102.00 | < 0.0001 | Significant |
A—% Organic modifier | 0.0026 | 1 | 0.0026 | 193.89 | < 0.0001 | |
B—pH | 0.0001 | 1 | 0.0001 | 7.43 | 0.0415 | |
C—Flow rate | 0.0047 | 1 | 0.0047 | 353.18 | < 0.0001 | |
For T. plate | ||||||
Model | 1.035E+07 | 9 | 1.149E+06 | 224.24 | < 0.0001 | Significant |
A—% Organic modifier | 46,665.12 | 1 | 46,665.12 | 9.10 | 0.0295 | |
B—pH | 23,653.12 | 1 | 23,653.12 | 4.61 | 0.0844 | |
C—Flow rate | 8.921E+06 | 1 | 8.921E+06 | 1740.34 | < 0.0001 | |