Table 1 siRNA’s with target sequences predicted by siDirect webserver. Here all these siRNA’s follows the all three rules of siRNA selection, e.g., Ui-Tei, Reynolds and Amarzguioui

S1

ATGTTTGTTTTTCTTGTTTTATT

1–23

UAAAACAAGAAAAACAAACAU

GUUUGUUUUUCUUGUUUUAUU

U R A

5.6 °C

5.6 °C

S2

TGGATTTTTGGTACTACTTTAGA

304–326

UAAAGUAGUACCAAAAAUCCA

GAUUUUUGGUACUACUUUAGA

U R A

9.8 °C

− 3.3 °C

S3

CGCTACTAATGTTGTTATTAAAG

360–382

UUAAUAACAACAUUAGUAGCG

CUACUAAUGUUGUUAUUAAAG

U R A

1.4 °C

6.3 °C

S4

GGGAATTTGTGTTTAAGAATATT

554–576

UAUUCUUAAACACAAAUUCCC

GAAUUUGUGUUUAAGAAUAUU

U R A

6.9 °C

5.3 °C

S5

AAGAATATTGATGGTTATTTTAA

568–590

AAAAUAACCAUCAAUAUUCUU

GAAUAUUGAUGGUUAUUUUAA

U R A

− 0.3 °C

− 1.8 °C

S6

GACTTTTCTATTAAAATATAATG

810–832

UUAUAUUUUAAUAGAAAAGUC

CUUUUCUAUUAAAAUAUAAUG

U R A

− 8.0 °C

7.1 °C

S7

TTCTATTAAAATATAATGAAAAT

815–837

UUUCAUUAUAUUUUAAUAGAA

CUAUUAAAAUAUAAUGAAAAU

U R A

8.9 °C

− 7.5 °C

S8

ATCTATCAAACTTCTAACTTTAG

925–947

AAAGUUAGAAGUUUGAUAGAU

CUAUCAAACUUCUAACUUUAG

U R A

9.8 °C

8.9 °C

S9

TTGTTAGATTTCCTAATATTACA

968–990

UAAUAUUAGGAAAUCUAACAA

GUUAGAUUUCCUAAUAUUACA

U R A

− 8.0 °C

6.9 °C

S10

CCCTTTTGATGAAGTTTTTAACG

999–1021

UUAAAAACUUCAUCAAAAGGG

CUUUUGAUGAAGUUUUUAACG

U R A

0.0 °C

7.4 °C

S11

CTGGAAATATTGCTGATTATAAT

1235–1257

UAUAAUCAGCAAUAUUUCCAG

GGAAAUAUUGCUGAUUAUAAU

U R A

8.7 °C

1.8 °C

S12

GACCTAAAAAGTCTACTAATTTG

1568–1590

AAUUAGUAGACUUUUUAGGUC

CCUAAAAAGUCUACUAAUUUG

U R A

6.3 °C

− 3.8 °C

S13

GTCTACTAATTTGGTTAAAAACA

1578–1600

UUUUUAACCAAAUUAGUAGAC

CUACUAAUUUGGUUAAAAACA

U R A

0.0 °C

6.3 °C

S14

TTGCAATATGGCAGTTTTTGTAC

2251–2273

ACAAAAACUGCCAUAUUGCAA

GCAAUAUGGCAGUUUUUGUAC

U R A

5.6 °C

5.6 °C

S15

CAGTTTTTGTACACAATTAAAAC

2262–2284

UUUAAUUGUGUACAAAAACUG

GUUUUUGUACACAAUUAAAAC

U R A

− 1.4 °C

5.6 °C

S16

GTGCAATTTCAAGTGTTTTAAAT

2903–2925

UUAAAACACUUGAAAUUGCAC

GCAAUUUCAAGUGUUUUAAAU

U R A

7.2 °C

7.4 °C

S17

TTGTAAACATTCAAAAAGAAATT

3518–354

UUUCUUUUUGAAUGUUUACAA

GUAAACAUUCAAAAAGAAAUU

U R A

5.5 °C

6.9 °C