In silico prediction of siRNA to silence the SARS-CoV-2 omicron variant targeting BA.4, BA.5, BQ.1, BQ1.1. and XBB: an alternative to traditional therapeutics

Future Journal of Pharmaceutical Sciences

Table 2 Calculation of free energy of binding, heat capacity, concentration plot and conservancy search against other sub-lineages of Sars-CoV2 virus

Alias	Target position in mRNA of Spike	Target Sequence (21nt target + 2nt overhang)	RNA oligo sequences 21nt guide (5′ → 3′) 21nt passenger (5′ → 3′)	Free energy of binding	Tm		Validity	% of RNA sequence matched with sub-lineages
Alias	Target position in mRNA of Spike	Target Sequence (21nt target + 2nt overhang)		Free energy of binding	Tm(Cp)	Tm (Conc)	Validity	% of RNA sequence matched with sub-lineages
S2	304–326	TGGATTTTTGGTACTACTTTAGA	UAAAGUAGUACCAAAAAUCCA GAUUUUUGGUACUACUUUAGA	− 30.2	81.4	80.2	89.5	100% (59/59)
S10	999–1021	CCCTTTTGATGAAGTTTTTAACG	UUAAAAACUUCAUCAAAAGGG CUUUUGAUGAAGUUUUUAACG	− 28.4	76.3	75.1	78.2	96.61% (57/59)