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Table 1 Predicted combination of factors as per central composite design

From: Development of a robust and reliable reverse-phase high-performance liquid chromatography (RP-HPLC) method using analytical quality by design principles for the accurate determination of esculin in its bulk form

Run

X1

(% of Methanol)

X2

(Flow rate)

R1

(Retention time)

R2

(Theoretical plates)

1

35

0.8

5.668

3700.34

2

35

1.2

4.11

2621.63

3

47.5

1.2

2.25

1674.26

4

47.5

1

2.9

2371.76

5

60

0.8

4.58

5632.22

6

60

1

3.38

4221.12

7

60

1.2

2.579

3272.73

8

47.5

1

3

2369.79

9

47.5

1

2.93

2400.86

10

35

1

4.88

3119.34

11

47.5

0.8

3.921

3330.04