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Table 2 Two-way ANOVA for Quadratic model

From: Development of a robust and reliable reverse-phase high-performance liquid chromatography (RP-HPLC) method using analytical quality by design principles for the accurate determination of esculin in its bulk form

Response

P value

Model significance

Polynomial equation

R1

0.0209

Significant

\(=2.95-0.6865\times A-0.8717\times B-0.1107\times AB+1.17\times {A}^{2}+0.1222{\times B}^{2}\)

R2

0.0001

Significant

\(=2377.61+614.13\times A-849\times B-320.20\times AB+1297.40\times {A}^{2}+129.32\times {B}^{2}\)