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Future Journal of Pharmaceutical Sciences

Table 4 Optimized run

From: Development of a robust and reliable reverse-phase high-performance liquid chromatography (RP-HPLC) method using analytical quality by design principles for the accurate determination of esculin in its bulk form

Parameter

Observations

Predicted values

Observed values

% RSD

Methanol (%) in mobile phase

43.2

43

0.328886875

Flow rate (ml/min)

0.901

0.9

0.07856742

Retention time (min)

3.77

3.78

0.187065286

Theoretical plates

2719.02

2727.43

0.218035588

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