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Future Journal of Pharmaceutical Sciences

Table 5 Optimized chromatographic conditions

From: Development of a robust and reliable reverse-phase high-performance liquid chromatography (RP-HPLC) method using analytical quality by design principles for the accurate determination of esculin in its bulk form

Parameters

Conditions

Flow rate

0.9 ml/min

Temperature

35 °C

Ratio

43:57 (Methanol:0.1% OPA)

Injection volume

10 µl

Retention time (%RSD, n = 6)

3.78 min

Area (%RSD, n = 6)

209,221

Tailing factor

1.336

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