Table 6 Summary of validation parameters
Validation parameters | Results |
|---|---|
Linearity | |
Correlation coefficient | 0.9998 |
Range | 4–20 µg/ml |
Sensitivity | |
LOD (µg/ml) | 0.82891 |
LOQ (µg/ml) | 2.511 |
Precision | |
Inter-day (%RSD) | 0.665 |
Intra-day (%RSD) | 0.612 |
Robustness | 0.9089 |
Flow rate (%RSD) | 0.7385 |
Ratio (%RSD) | 0.9365 |
Temperature (%RSD) | 1.0516 |
Ruggedness | |
Change in analyst (%RSD) | 1.1011 |
System suitability | 1.24 |
Retention time (%RSD) | 0.1398 |
Peak area (%RSD) | 0.7146 |
Theoretical plates (%RSD) | 0.0612 |