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Future Journal of Pharmaceutical Sciences
Fig. 15 | Future Journal of Pharmaceutical Sciences

Fig. 15

From: Neuroprotective action of Smilax china ethanolic bark extract in treatment of a prominent aging disorder: Parkinson’s disease induced by rotenone

Fig. 15

Thin-layer chromatography (TLC) is used for the chromatographic analysis. To pinpoint the origin of the two discolorations, iodine vapor was sprayed into the area. Scanning at 254 nm, 365 nm, and visible light. Toluene/ethyl acetate/acetic acid (5:4:1) used as the mobile phase. Extracts used during TLC were 1—ethanol extract, 2—water fraction, 3—ethyl acetate fraction, and 4—N-hexane fraction which were viewed under Ultraviolet 254 nm, Ultraviolet 365 nm, and visible light. Multiple spots were seen in a thin layer of prepared silica gel-G on a chromatographic plate

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