Table 1 Purification of Methioninase from Methylobacterium sp. JUBTK33
Purification | Enzyme activity (U/mL/min) | Total protein content (U/mL) | Specific activity (U/mg) | Purification fold |
|---|---|---|---|---|
Crude sample | 1.26 ± 0.12 | 700 | 2.91 | 1 |
SPMet’s—1 mg/mL | 1.5 ± 0.91 | 119 | 12.60 | 4.30 |
DEAE column purified | 0.9 ± 0.11 | 61 | 14.75 | 5.07 |
Sephadex G-200 column purified | 0.34 ± 0.14 | 19 | 17.89 | 6.15 |