Table 1 Validation parameters of the UHPLC method for quantification of SOF, LDV and PAR in bulk

Range(μg/ml)

5–60

2–22

1–22

Slope

13.39

13.99

61.25

Intercept

− 2.783

− 18.41

58.26

Correlation coefficient(r)

0.9993

0.9998

0.9998

Accuracy (mean ± SD)

99.99 ± 0.554

99.11 ± 0.197

100.362 ± 0.535

Precision (RSD %)

 Intra-day*

0.884

0.938

0.702

 Inter-day*

0.776

0.403

0.355

 LOD (μg/ml)**

0.153

0.074

0.015

 LOQ(μg/ml)***

0.464

0.225

0.045