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Table 6 System suitability parameters for quantification of SOF, LDV and PAR

From: Rapid and validated UHPLC method for simultaneous determination of sofosbuvir, ledipasvir and paracetamol as commonly repurposed drugs for COVID-19 treatment: application in spiked human plasma

Parameter

Value

Reference value

SOF

LDV

PAR

 

Retention time(min)

3.2

2.3

1.7

 

Capacity factor (k’)

9.65

7.98

5.55

1–10

Selectivity (α)

1.46

1.44

1.26

α > 1

Resolution (Rs)

6.51

3.68

1.5

R ≥ 1.5

Tailing factor(T)

1.356

1.875

1.184

1–2

Number of Theoretical plates

13,314.2

2622.07

1793.7

Increase with efficiency of separation

HETP*

0.01

0.05

0.08

The smaller the value the higher the column efficiency

  1. *Height equivalent to theoretical plates = column length/Number of Theoretical plates