Table 1 Summary results noticed in method validation

System suitability^$

t_R (min)

6.91

2.89

1.91

RRT

–

0.42

0.28

RRF

–

0.110

0.142

R_S

14.25

3.88

–

A_S

0.92

0.96

0.93

N

8255

4469

3670

Linearity

Range in µg/mL

30–180

0.03–0.18

0.03–0.18

Slope

8587.3

739,310

995,909

Intercept

−158,895

−1329.1

−5945.6

r²

0.9956

0.9965

0.9994

Precision^$$

Intraday

0.54

1.38

0.40

Interday (day 1)

0.66

0.88

0.16

Interday (day 2)

0.58

0.53

0.17

LOQ level

–

1.18

0.96

Accuracy at 50% level^$

Amount added (µg/mL)

90

0.09

0.09

Recovered (µg/mL)

88.99

0.089

0.090

% Recovery

98.87

98.99

99.72

% RSD

0.20

0.48

0.32

Accuracy at 100% level^$

Amount added (µg/mL)

120

0.12

0.12

Recovered (µg/mL)

118.89

0.119

0.120

% Recovery

99.07

99.16

99.76

% RSD

0.83

0.66

0.57

Accuracy at 150% level^$

Amount added (µg/mL)

150

0.15

0.15

Recovered (µg/mL)

149.64

0.146

0.150

% Recovery

99.76

97.60

99.82

% RSD

0.65

1.46

0.56

Sensitivity

LOD (µg/mL)

–

0.009

0.009

LOQ (µg/mL)

–

0.03

0.03