Table 1 Gradient elution and gradient flow program
Time (min) | 0.1% OPA buffer (%) | Acetonitrile (%) | Flow rate (ml/min) |
|---|---|---|---|
0 | 100 | 0 | 1.0 |
1 | 97 | 3 | 1.0 |
10 | 95 | 5 | 1.0 |
12 | 85 | 15 | 1.5 |
16 | 75 | 25 | 1.5 |
20 | 55 | 45 | 1.0 |
24 | 40 | 60 | 1.0 |
24.5 | 20 | 80 | 1.0 |
28 | 0 | 100 | 1.0 |
28.5 | 97 | 3 | 1.0 |
35 | 97 | 3 | 1.0 |