Table 9 Results of HPLC validation
Validation parameter | Catechin 280 nm | Epicatechin 280 nm | Epigallocatechin 274 nm | Ellagic acid 254 nm |
|---|---|---|---|---|
Specificity-Rt UV peak purity | 8.2 99% | 10.8 99% | 11.6 99% | 15.2 98% |
Linearity Conc. Range R2 | ||||
10–50 ppm | ||||
0.99911 | 0.99915 | 0.99954 | 0.99965 | |
LOD-ppm | 1.58 | 2.01 | 0.93 | 1.48 |
LOQ-ppm | 4.81 | 6.09 | 2.84 | 4.5 |
Precision | ||||
Intraday area precision RSD | 1.03% | 0.69% | 0.21% | 0.49% |
Area response factor Concentration/Avg. response | 14.9 | 19.9 | 9 | 225.4 |