Table 3 Specificity of API and its related substances
Retention time (major peak) | |||||
|---|---|---|---|---|---|
ID | HC | HCB | HCB-21 | HC-3-methyl enol ether 17-butyrate | HCB-17,21-methylorthobutyrate |
Diluent | |||||
Working Std -1 | 2.89 min | 8.43 min | 9.14 min | ||
HCB | 2.89 min | ||||
HCB-21 | 9.14 min | ||||
HC-3-methyl enol ether 17-butyrate | 13.70 min | ||||
HC-17,21-methylorthobutyrate | 16.25 min | ||||
All ID Mixture | 2.89 min | 8.43 min | 9.15 min | 13.70 min | 16.25 min |