Fig. 4
From: Peptide-modified PAMAM-based bone-targeting RNA delivery system
Gene silencing ability of PS nanoparticles in HEK293-EGFP cells transfected with GFP siRNA. A Fluorescent microscopy images of HEK293-EGFP cells transfected with GFP siRNA using P, Lipofectamine™ 2000 (Lipo™ 2000), and PEI. Lipofectamine™ 2000 (Lipo™ 2000), and PEI-treated groups served as positive controls (scale bar: 50 µm). B Semi-quantified analysis of green channel images using ImageJ software. Data are represented as mean ± SD, n = 3. A student’s t-test was used to compare the differences between the two groups. *P < 0.05, **P < 0.01 vs. blank. (P + siRNA: PAMAM + GFP siRNA; PS + RNA: SDSSD-modified PAMAM + GFP siRNA; Lipo + siRNA: Lipofectamine™ 2000 + GFP siRNA; PEI + siRNA: Polyethylenimine + GFP siRNA; ns: not significant)