Fig. 5
From: Peptide-modified PAMAM-based bone-targeting RNA delivery system
In vitro targeting and delivery ability of PS nanoparticles. A Relative expression of miR-138-5p and B its target gene, MACF1, in the MC3T3-E1 cell line. C Relative expression of miR-138-5p and D its target gene, MACF1, in the RAW 264.7 cell line. U6 and GAPDH were the internal controls for 138-5p and MACF1, respectively. Data are shown as mean ± SD, n = 3. A student’s t-test was used to compare the differences between the two groups. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 vs. P + RNA. (RNA: Antagomir 138-5p; P + RNA: PAMAM (without SDSSD) + Antagomir 138-5p; PS + RNA: SDSSD-modified PAMAM + Antagomir 138-5p; Anti-miRNA: Antagomir 138-5p; ns: not significant)