Table 4 Intra-day precision values at different levels of the validated analytical method
Analytes | Actual concentration (µg/mL) | Observed Concentration* (µg/mL) | Intra-day precision %RSD | % Accuracy* | Avg. % Bias |
|---|---|---|---|---|---|
BCA | 0.5 | 0.495 (± 0.006) | 1.130 | 98.998 (± 1.12) | 1.002 |
4 | 3.98 (± 0.044) | 1.102 | 99.499 (± 1.1) | 0.5015 | |
32 | 31.812 (± 0.619) | 1.947 | 99.413 (± 1.94) | 0.5874 | |
LTB | 0.5 | 0.518 (± 0.007) | 1.403 | 103.86 (± 1.455) | − 3.7763 |
4 | 3.954 (± 0.07) | 1.927 | 98.86 (± 1.905) | 1.1392 | |
32 | 31.84 (± 0.573) | 1.800 | 99.51 (± 1.791) | 0.4894 |