Table 5 Inter-day precision values at different levels of the validated analytical method
Analytes | Actual concentration (µg/mL) | Observed concentration* | Inter-day precision %RSD | % Accuracy* | Average bias |
|---|---|---|---|---|---|
BCA (Day 1) | 0.5 | 0.484 (± 0.006) | 1.154 | 96.862 (± 1.11) | 3.13 |
4 | 3.916 (± 0.044) | 1.120 | 97.89 (± 1.09) | 2.10 | |
32 | 31.22 (± 0.207) | 0.662 | 97.563 (± 0.64) | 2.43 | |
LTB (Day 1) | 0.5 | 0.512 (± 0.007) | 1.421 | 102.46 (± 1.45) | − 2.46 |
4 | 3.915 (± 0.076) | 1.946 | 97.87 (± 1.90) | 2.12 | |
32 | 31.209 (± 0.573) | 1.837 | 97.52 (± 1.79) | 2.47 | |
BCA (Day 2) | 0.5 | 0.4740 (± 0.005) | 1.180 | 94.501 (± 1.11) | 5.18 |
4 | 3.95 (± 0.043) | 1.109 | 98.87 (± 1.097) | 1.12 | |
32 | 30.80 (± 0.1514) | 0.491 | 96.271 (± 0.47) | 3.72 | |
LTB (Day 2) | 0.5 | 0.506 (± 0.007) | 1.438 | 101.21 (± 1.45) | − 1.21 |
4 | 3.939 (± 0.076) | 1.934 | 98.479 (± 1.90) | 1.52 | |
32 | 31.04 (± 0.573) | 1.847 | 97.004 (± 1.79) | 2.99 | |
BCA (Day 3) | 0.5 | 0.494 (± 0.005) | 1.131 | 98.86 (± 1.11) | 1.13 |
4 | 3.958 (± 0.043) | 1.108 | 98.96 (± 1.09) | 1.03 | |
32 | 32.15(± 0.209) | 0.650 | 100.48 (± 0.63) | − 0.48 | |
LTB (Day 3) | 0.5 | 0.518 (± 0.072) | 1.404 | 103.69 (± 1.45) | − 3.69 |
4 | 3.9414 (± 0.076) | 1.933 | 98.536 (± 1.90) | 1.46 | |
32 | 30.816 (± 0.282) | 0.918 | 96.30 (± 0.88) | 3.69 |