Table 1 Optimized chromatographic conditions of current method

Mobile Phase

0.1% OPA in water: Acetonitrile (70:30 v/v)

Column

HSS C18 (100 × 2.1 mm, 5 µm)

Flow Rate

0.3 mL/min

Temperature

Ambient

Volume

2µL

Wavelength

230 nm

Diluent

Water: Acetonitrile (50:50)

Retention time

Olanzapine – 0.46 min

Samidorphan – 0.93 min