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Table 2 Analytical/statistical data from standard curves and quantification of bitter acids and xanthohumol present in hops

From: Inhibitory capacity of extracts and main constituents of hop flowers

Compound

λ (nm)

Rt ≈ (min)

Standard curve range (mg/mL) n = 7

Standard curve equations

R2

Commercial pellets g/100 g

Cones (Brazil)

Matured hop g/100 g

First Bloom g/100 g

Saaz n = 3 (RSD%)

Herkules n = 3 (RSD%)

Chinook-1 n = 3 (RSD%)

Chinook-2 n = 3 (RSD%)

Cascade n = 3 (RSD%)

Xanthohumol (XN)

340

5.7

0.006–0.18 (n = 6)

y = 44.97 × + 67.39

0.999

0.010 (12.86)

2.165 (4.90)

2.402 (5.51)

0.432 (16.98)

0.083 (13.57)

Cohumulone (α1)

326

12.8

0.027–0.432

y = 10,533 × − 4.4493

0.991

0.675 (3.14)

2.810 (4.03)

1.360 (11.57)

0.345 (10.25)

0.327 (15.23)

Humulone + Adhumulone (α2)

326

14.1

0.079–1.264

y = 10,669 × − 79.51

0.997

1.172 (6.04)

6.045 (1.29)

5.054 (17.31)

0.945 (20.20)

0.386 (18.76)

Colupulone (β1)

326

15.2

0.032–0.576

y = 7,785 × + 88.62

0.996

1.260 (7.86)

1.405 (21.47)

0.144 (9.43)

0.355 (17.92)

0.145 (13.54)

Lupulone + Adlupulone (β2)

326

16.0

0.034–0.538

y = 10,159 × − 40.358

0.993

0.985 (7.90)

1.290 (16.55)

0.092 (8.99)

0.375 (14.51)

0.166 (14.99)

Total

4.102

13.715

9.052

2.452

1.107

  1. This table should be added after the fourth paragraph of the section “Qualitative and quantitative analysis of chemical constituents”
  2. λ—analysis wavelength (nm), Rt—retention time (min), RSD—relative standard deviation, R2—determination coefficient