Table 1 Optimised liquid chromatography and mass spectroscopic conditions

Isocratic step mobile

ACN: Formic acid 0.1% in water 50:50 v/v

Ionisation source

Drying gas: N₂ gas@Drying flow rate: 5 ml/min@Pressure: 55 psi

Flow level: 1 ml/min

Source temperature: 550 °C

Injection volume: 10 µl

Capillary voltage: 5500 V

Inertsil ODS

250 mm length

Collision cell gas

Nitrogen with high purity

4.6 mm ID

Mode

MRM^b

5 µm PS

Analyte

Olanzapine

Olanzapine MRM transitions

m/z-313.40 → m/z-192.54@CE^a—14 V

Samidorphan

Samidorphan MRM transitions

m/z-371.45 → m/z220.61@CE^a—15 V

Internal standard

D₃-Olanzapine

D₃-Olanzapine MRM transitions

m/z-316.40 → m/z-237.58@CE^a—14 V

D₃-Samidorphan

D₃-Samidorphan MRM transitions

m/z-374.41 → m/z-223.61@CE^a—15 V