Stability | Storage condition | Conc. level | Quantified conc. (ng/ml) (Mean ± SD, n = 6) | % RSD | % Recovery |
---|---|---|---|---|---|
Bench top stability | 18 h at room temperature | 10 | 10.313 ± 5.1 | 1.54 | 99.54 |
20 | 20.207 ± 1.6 | 0.37 | 98.96 | ||
30 | 30.421 ± 2.0 | 0.59 | 99.42 | ||
Autosampler stability | 24 h in autosampler at room temperature | 10 | 10.078 ± 5.7 | 2.54 | 98.75 |
20 | 20.112 ± 5.4 | 0.24 | 99.22 | ||
30 | 30.328 ± 3.6 | 1.64 | 99.32 | ||
Long-term stability (Day 28) | 28 days at (− 20 ± 3)°C | 10 | 9.649 ± 4.8 | 2.22 | 85.76 |
20 | 20.334 ± 6.1 | 0.58 | 86.26 | ||
30 | 30.293 ± 20.0 | 0.94 | 87.54 | ||
Freeze thaw stability | 24 h at (28 ± 5)°C then exposed to three freeze and thaw cycles | 10 | 10.498 ± 5.3 | 2.47 | 98.85 |
20 | 19.485 ± 7.4 | 1.36 | 99.45 | ||
30 | 29.554 ± 6.7 | 0.94 | 98.51 | ||
Wet extract stability | 18 h at 2–8 °C | 10 | 10.368 ± 2.8 | 0.85 | 98.32 |
20 | 20.491 ± 5.8 | 0.42 | 98.85 | ||
30 | 30.589 ± 3.4 | 0.67 | 99.59 | ||
Dry extract stability | 18 h at (− 20 ± 3)°C | 10 | 10.647 ± 7.8 | 2.54 | 99.48 |
20 | 19.328 ± 9.5 | 1.39 | 99.54 | ||
30 | 30.514 ± 4.4 | 1.28 | 99.83 | ||
Short-term stability | 7 days at (5 ± 3)°C | 10 | 9.272 ± 7.6 | 0.42 | 94.65 |
20 | 20.367 ± 2.8 | 2.51 | 95.74 | ||
30 | 30.604 ± 12.6 | 3.84 | 94.59 |