Table 2 Formulation constraints and responses for CCD
Batch No | Concentration of lipid blend (% w/w) (X1) | Surfactant (% w/v) (X2) | P-size (nm) (Y1) | PdI (Y2) | Zeta Potential (ζ) (mV) (Y3) |
|---|---|---|---|---|---|
1 | 6 | 4 | 346.3 | 0.416 |  − 31.6 |
2 | 4 | 3 | 249 | 0.371 |  − 31.2 |
3 | 4 | 3 | 244.3 | 0.341 |  − 29.8 |
4 | 4 | 3 | 236 | 0.363 |  − 29.6 |
5 | 4 | 3 | 225 | 0.354 |  − 31.4 |
6 | 2 | 3 | 266 | 0.408 |  − 28.8 |
7 | 2 | 2 | 346.8 | 0.467 |  − 25.3 |
8 | 4 | 3 | 256 | 0.38 |  − 29.2 |
9 | 4 | 4 | 144 | 0.301 |  − 33.2 |
10 | 4 | 2 | 311 | 0.432 |  − 26.8 |
11 | 6 | 3 | 460 | 0.47 |  − 27.8 |
12 | 6 | 2 | 520 | 0.508 |  − 26.9 |
13 | 2 | 4 | 244 | 0.361 |  − 32.3 |
Scale up | 20 | 20 | 130 | 0.279 |  − 33 |