Volumes | Mean area ± RSD |
---|---|
Mobile phase compositions (Hexane: Ethyl acetate, v/v) | |
6.9:3.1 | 695 ± 2.20 |
7.1:2.9 | 698 ± 1.90 |
7:3 | 701 ± 2.42 |
Temperature (°C) | |
22 ± 2 | 702 ± 2.80 |
25 ± 2 | 706 ± 2.00 |
27 ± 2 | 704 ± 2.65 |
Humidity (% RH) | |
45 ± 5 | 704 ± 2.35 |
55 ± 5 | 708 ± 2.70 |
65 ± 5 | 706 ± 2.50 |
Distance for development of TLC plate (mm) | |
78 | 720 ± 2.10 |
76 | 726 ± 1.80 |
74 | 723 ± 2.20 |
Wavelength (nm) | |
287 | 715 ± 1.40 |
285 | 725 ± 1.70 |
283 | 720 ± 1.90 |