Table 11 Data of robustness studies of koenimbine
Volumes | Mean area ± RSD |
|---|---|
Mobile phase compositions (Hexane: Ethyl acetate, v/v) | |
6.9:3.1 | 756 ± 1.32 |
7.1:2.9 | 760 ± 1.40 |
7:3 | 764 ± 2.10 |
Temperature (°C) | |
22 ± 2 | 758 ± 2.30 |
25 ± 2 | 762 ± 2.10 |
27 ± 2 | 760 ± 1.95 |
Humidity (% RH) | |
45 ± 5 | 768 ± 1.20 |
55 ± 5 | 776 ± 2.20 |
65 ± 5 | 772 ± 1.80 |
Distance for development of TLC plate (mm) | |
78 | 764 ± 2.40 |
76 | 770 ± 2.10 |
74 | 767 ± 2.30 |
Wavelength (nm) | |
293 | 774 ± 1.60 |
291 | 782 ± 2.10 |
289 | 774 ± 2.20 |