Table 3 Method validation parameters
Sl No | Parameters | Mahanimbine | Koenimbine |
|---|---|---|---|
1 | Wavelength | 285 nm | 291 nm |
2 | Linearity range concentration (in µg/ml) | 100–400 | 50–450 |
3 | Coefficient of variances (CV) | 0.34% | 0.89% |
4 | Correlation coefficients (R2) | 0.9985 | 0.9998 |
5 | Limit of detection (ng/spot) | 32.81 | 18.44 |
6 | Limit of quantitation (ng/spot) | 72.81 | 31.57 |
7 | Reproducibility | Reproducible | Reproducible |