Table 1 The primer sequences
From: Cordycepin alleviates osteoarthritis by inhibiting chondrocyte ferroptosis via Keap1/Nrf2 axis
Name | Primer | Sequence |
|---|---|---|
β-actin | Forward | 5’- TGTCCACCTTCCAGCAGATGT-3’ |
Reverse | 5’- AGCTCAGTAACAGTCCGCCTAGA-3’ | |
Keap1 | Forward | 5’- TGGACTTTCGTAGCCTCCAT-3’ |
Reverse | 5’- GCATTCCACACTGTCCAGAA-3’ | |
Nrf2 | Forward | 5’- CAGCATAGAGCAGGACATGGAG-3’ |
Reverse | 5’- GAACAGCGGTAGTATCAGCCAG-3’ | |
GPX4 | Forward | 5’- CCTCTGCTGCAAGAGCCTCCC-3’ |
Reverse | 5’- CTTATCCAGGCAGACCATGTGC-3’ | |
SLC7A11 | Forward | 5’- CTTTGTTGCCCTCTCCTGCTTC-3’ |
Reverse | 5’- CAGAGGAGTGTGCTTGTGGACA-3’ | |
MMP13 | Forward | 5’- GCATTGGCTGAGTGAAAGAGAC-3’ |
Reverse | 5’- ATGATGAACGATGGACAGATGA-3’ | |
ADAMTS-5 | Forward | 5’- ATGATTCGCCTCGGGGCTC-3’ |
Reverse | 5’- GCACTCTCCGAAGGGGATCT-3’ | |
Col2a1 | Forward | 5’- GTGGAGGTGGACGCTACACTCA-3’ |
Reverse | 5’- AGCCAGGTTGCCATCGCCATA-3’ | |
aggrecan | Forward | 5’- ACCAGACTGTCAGATACCCC-3’ |
Reverse | 5’- CATAAAAGACCTCACCCTCC-3’ |