Table 2 The antibodies for western blotting
From: Cordycepin alleviates osteoarthritis by inhibiting chondrocyte ferroptosis via Keap1/Nrf2 axis
Classification | Name | Manufacturer | Catalog number | Dilution ratio |
|---|---|---|---|---|
Primary antibodies | β-actin | Abcam | ab227387 | 1:1000 |
Keap1 | Abcam | ab119403 | 1:1000 | |
Nrf2 | Affinity | AF7006 | 1:1000 | |
GPX4 | Sigma-Aldrich | SAB5700944 | 1:1000 | |
SLC7A11 | KALANG | kl410Ra21 | 1:1000 | |
MMP13 | Abcam | ab219620 | 1:1000 | |
ADAMTS-5 | Abcam | ab41037 | 1:1000 | |
Col2a1 | Affinity | AF5456 | 1:1000 | |
aggrecan | Abcam | ab313636 | 1:1000 | |
Secondary antibodies | HRP Conjugated AffiniPure Goat Anti-rabbit IgG (H + L) | Boster | BA1054 | 1:50000 |