Table 3 Summary of validation parameter
S. no. | Validation parameters | Quercetin | Piperine |
|---|---|---|---|
1 | Linearity | ||
|  |  Linearity range (µg/mL) | 2–10 | 2–10 |
|  |  Correlation-coefficient |  < 0.999 |  < 0.998 |
2 |  LOD (µg/mL) | 0.32 | 0.41 |
|  |  LOQ(µg/mL) | 0.98 | 1.32 |
3 | Precision | ||
|  |  Intra-day (%RSD) | 0.521 ± 0.07 | 0.2 ± 0.003 |
|  |  Inter-day (%RSD) | 1.25 ± 0.027 | 0.42 ± 0.01 |
4 | Robustness | ||
|  |  Change in Mobile phase volume (%RSD) | 0.710 ± 0.047 | 0.28 ± 0.041 |
|  |  Change in flow rate (%RSD) | 0.323 ± 0.02 | 0.83 ± 0.018 |
5 | Accuracy | ||
|  |  50% recovery | 98.75 ± 0.02 | 101.31 ± 0.24 |
|  |  100% recovery | 100.24 ± 0.37 | 100.78 ± 0.51 |
|  |  150% recovery | 98.21 ± 0.52 | 99.21 ± 0.23 |