Table 2 Preparation of standards
Stock solution (ng/ml) | Volume taken (µl) | Made up to volume (µl) | Final concentration (ng/ml) | Identification |
|---|---|---|---|---|
200 | 50 | 2000 | 5.0 | Standard 1 |
200 | 125 | 2000 | 12.5 | Standard 2 |
200 | 250 | 2000 | 25.0 | Standard 3 |
200 | 375 | 2000 | 37.5 | Standard 4 |
200 | 500 | 2000 | 50.0 | Standard 5 |
200 | 625 | 2000 | 62.5 | Standard 6 |
200 | 750 | 2000 | 75.0 | Standard 7 |
200 | 1000 | 2000 | 100.0 | Standard 8 |