Detection of 6-monoacetylemorphine in hair sample of heroin addicts using gas chromatography–mass spectrometry and significance of rehabilitation program

Future Journal of Pharmaceutical Sciences

Table 1 Calibration method for sample

Groups	Conc ng/mg	t_R min	Slope (mean± S.D)	y-intercept	Linearity^a (R²)	LOD^b (ng/mg)	LLOQ^c (ng/mg)
Addicts’ group	0.1–25	7.23	0.334±0.010	0.0572	0.9984	0.0745	0.22
Rehab group	0.1–25	7.22	0.265±0.032	0.0412	0.9937	0.0311	0.09

^aLinearity is described by the determination coefficient for the calibration curve (y=ax+b). ^bThe limit of detection (LOD) was based on the concentration corresponding to a signal plus 3 standard deviations from the mean of 10 replicates of the blank hair. ^cThe lower limit of quantification (LLOQ) was defined as the lowest concentration on the calibration curve with precision (CV%) less than 20% and accuracy (bias%) within ±20%