Detection of 6-monoacetylemorphine in hair sample of heroin addicts using gas chromatography–mass spectrometry and significance of rehabilitation program
Future Journal of Pharmaceutical Sciences volume 7, Article number: 98 (2021)
The analysis of hair samples for the detection of drugs has become one of the convincing strategies in the field of forensic toxicology. A large number of cases concerning heroin abuse or its byproducts have been documented under the Control of Narcotic Substances Act, 1997, over the past decade. The present study was carried out with an aim to evaluate the primary metabolite of heroin, i.e., 6-monoacetylemorphine (6-MAM), in hair samples of addicts and subjects undergoing rehabilitation, thereafter accessing the success rate of the rehabilitation program at the de-addiction center.
Hair samples were randomly collected from 20 regular heroin addicts and 20 heroin addicts from their past, from the rehabilitation center, of different age groups. Samples were cleaned, digested, and extracted using an alkaline digestion mediator methyl tertiary butyl ether, followed by quantification of 6-MAM via gas chromatography–mass spectrometry (GC–MS). The mean concentration of 6-MAM in regular heroin addicts detected was 7.80 ng/mg and 2.34 ng/mg in samples of subjects undergoing rehabilitation at the de-addiction center, respectively.
Traces of 6-MAM in the hair sample of heroin addicts can be efficiently detected days after the last intake of heroin. In addition to that, our findings also give an idea for future evaluating the approximate timeframe for detection of 6-MAM and/or other metabolites of heroin in the hair sample. However, in the future, by carefully analyzing the hair samples that can be taken from rehabilitation centers from target subjects at different time intervals, the exact duration of traceable quantity of 6-MAM can be determined in the hair sample. Finally, it can be concluded that there is a significant success rate of the rehabilitation program at de-addiction centers in connection with dragging the 6-MAM level from the body.
Heroin is a semisynthetic opiate, its chemical name is diacetylmorphine (DAM), and it shares close structural features with morphine. Although it is used as a strong analgesic agent around the world, in the USA, it is no longer used clinically . Similar to morphine and other poppy-derived agents, its relaxing and euphoric aptitude makes it a widespread drug of abuse. It is generally injected intravenously, even though it can be consumed by other means such as snorting, smoking, or inhaling its vapors. It shares the central structure of morphine, with the addition of 2 acetyl groups, which are believed to augment its permeation into the central nervous system . It is generally metabolized by sequential removal of these acetyl groups; the loss of the first acetyl group converts heroin into 6-monoacetylmorphine (6-MAM) . The 6-MAM is a primary metabolite of heroin, and its presence is a definitive indication of heroin abuse. Like heroin, 6-MAM has a very short half-life; however, 6-MAM is further metabolized into morphine, the dominant metabolite of heroin. The detection of 6-monoacetylmorphine establishes heroin exposure, but this metabolite has an extremely short half-life and is detectable in urine for only approximately 8 h after a single dose [3, 4]. Heroin is a controlled substance; it interrupts the brain and causes euphoria (sense of well-being), hallucination (changes the perception), and how one feels and how one’s body responds to pain sensation, making it a popular addictive drug. It is synthetically produced utilizing opium as its primary source. The manufacturing of heroin involves prior production of morphine, which is then chemically modified to obtain heroin . Among various ways of detecting the presence of drugs and/or their metabolites such as 6-MAM and morphine in a human body, one method that is being broadly used in recent days for forensic analysis is hair sampling of the suspect [6, 7]. Analysis of hair samples in the field of forensic science commenced in 1979 . Baumgartner and colleagues thrived in spotting opiates in the hair samples of heroin addicts by using radioimmunoassay (RIA) and reckoning their corresponding opiate abuse histories by sectional analysis. As far as decomposition of hair is concerned, it is not much decomposable, rather it is an astonishing organic matrix that protects organic molecules . It has the benefit, dependent upon its size, of permitting the expert to calculate the ingestion of a drug which might have taken place either a few days or several months before, and as an outcome, hair analysis is considered as a “timeline marker” for the former exposure to drugs . The number of drug-facilitated crimes in Pakistan has increased significantly over the past two decades. According to the National Police Bureau NPB Publication Titled “Crime Data and Analysis (2001-2015)” Pakistan, a large number of drug abuse cases have been registered under the “Control of Narcotic Substances Act, 1997” over the last decade and those pertaining to the abuse of heroin or their derivatives contribute the highest percentage [11, 12]. As has been witnessed by the authorities, in a number of cases, complaints against drug abusers were registered long after the quantity of drug ingested would have been eliminated naturally from the suspect’s blood or urine [13, 14]. The potential long-term historical record of drug exposure in hair could allow the detection of these otherwise short-lived analytes. Therefore, the present study was conducted with an aim to appraise the success rate of a de-addiction center by comparing the levels of 6-MAM traces in hair samples in regular heroin abusers and subjects (previously heroin addicts), undergoing rehabilitation at the de-addiction center, finally paving the way to develop a mechanism for estimating the retention time of 6-MAM in hair samples of addicts via gas chromatography–mass spectrometry (GC–MS) using an alkaline extracting agent methyl tertiary butyl ether (MTBE).
Chemicals and reagents
Dichloromethane (DCM), acetone, methyl tertiary butyl ether (MTBE), formic acid, and sodium hydroxide were all provided by the Department of Chemistry, Author University, Lahore, Pakistan, whereas Certified Reference Material (CRM) for internal standard deuterated 6-MAM, of purity ≥ 99%, was bought from Cayman Chemical (MI, USA).
After taking prior consent, 10–30 hair strands were taken from the scalps of each of the 20 random heroin addicts from a well-known area of Darbar Road at Lahore, Pakistan. A further 20 samples of subjects were taken, with prior consent, from Genius Rehab center situated at Barki Road, Lahore (Pakistan). The study approval was given by the Department of Chemistry and Life Sciences, Government College University, Lahore, with approval letter number, GCU/1-056/CLS. The participants were included after approval from the “Office of Research, Innovation and Commercialization” (ORIC) with reference number 2017-0057. All the participants were informed about the study protocol and then included after taking verbal consent. The inclusion criterion set for subjects at the rehabilitation center was those who have gone through at least 30 days of rehabilitation period against heroin abuse. The average duration of a patient at the rehabilitation center for sample collection, in our study, was 35 days. The hair samples from all participants were taken from the apex portion of the head, in order to avoid ambiguity in the dispersal of 6-MAM in hair due to inconstant rate of hair growth in different parts of the head (Tables 3 and 4).
Sample inclusion criteria
a) Only those individuals who had been admitted to the mentioned de-addiction center at least 30 days prior to the sample collection date were selected.
b) Healthy other than heroin addiction subjects of different age groups were included for this study.
Sample exclusion criteria
a) Individuals who got admitted to the mentioned de-addiction center against heroin abuse less than 30 days at the sample collection date were omitted from this study.
b) Individuals who had not taken heroin for at least 3 days (for addicts) prior to the date of sample collection were not considered for this study.
c) Individuals presenting any psychotic disorder or any other physical complaint or comorbid situation were excluded from the study.
d) Individuals who had experienced any kind of surgery, cosmetic hair treatment, or bald were excluded from this study.
Each round of hair analysis comprised of the following steps:
a) Sample decontamination
b) Digestion and extraction of the hair sample
c) Quantification of 6-MAM
Approximately, 50 mg of each sample was washed at room temperature, firstly with distilled water for 5 min, then with acetone for 1 min, and finally with dichloromethane for 2 min. The sample was air dried and then cut into small segments of about 3–4 mm .
Digestion and extraction of the hair sample
The washed samples were incubated with 500 μL of 1 M NaOH for 3 h in a water bath at 50 °C to allow for digestion. Samples were then extracted with 2 mL MTBE for 30-min rotary mixing and for 10-min centrifugation. Solvent layers were then shifted into clean tubes containing 150 μL 1% formic acid, then mixed on a rotary mixer for 20 min, and were removed after 10 min of centrifugation by aspiration. From the remaining aqueous layer, a 1-μL sample was injected into the GC–MS system [16,17,18].
Quantification of the 6-MAM
Shimadzu’s GC–MS-QP-2010 was used for the quantification of 6-MAM. The column was HP-5 crosslinked 5% phenylmethyl polysiloxane fused-silica capillary column (25 m (length) × 0.32 mm (internal diameter) × 0.17 μm (film thickness)). Helium of purity 99.9% was used as carrier gas. The temperature of the injector was 280 °C and the flow rate of the carrier gas was 1 mL/min. The temperature of the oven was maintained at 110 °C for 3 min at 10 °C/min, followed by 210 °C for 2 min at 10 °C/min, and finally at 300 °C for 5 min at 20 °C/min. SIM mode was used for quantitative analysis of 6-MAM (m/z <300) .
To evaluate selectivity, blank hair samples obtained from five different origins were analyzed to determine endogenous compounds or potential interferences released from the matrix. LOD and LLOQ were measured by evaluating the signal/noise (S/N) ratio of 10 replicates of blank hair for each compound at proper concentrations. LOD was calculated on the basis of the concentration with a S/N > 3, while the concentrations of the target compounds with a S/N > 10 were chosen as LLOQ. The absence of carry-over was evaluated by injecting at the highest point of the calibration curve, followed by a solvent blank, and measuring the peak area at the retention times of the target compounds under investigation. For routine analysis of hair samples, ethyl acetate blanks were run between each pair of samples. The linearity of the method was evaluated over the concentration ranges of 0.1–25 ng/mg for the sample in both groups and was expressed by the determination coefficient (R2). The calibration curves were obtained by least-squares linear regression. The intra-day precision and accuracy of the method were established by eight independent determinations of the samples (n=8). The inter-day precision and accuracy were determined in two different days for the aforementioned replicates over a 5-day gap for each replicate. To determine the precision, the coefficients of variations (CV%) were calculated for the replicate measurements. Accuracy (bias%) was expressed as the relative error of the calculated concentrations and was calculated by the degree of agreement between the measured and the nominal concentrations of the QC samples. For recovery determination, QC samples were prepared at three concentration levels. The recovery was determined by comparing the absolute peak area (A) of the compound for the QC samples prepared in three replicates with the absolute peak area (B) of the target compound for the samples processed as blank and spiked at the same concentration level. The recovery was calculated using the following equation: Recovery (%) = A/B × 100. Sample stability was assessed by repeated analysis of low-concentration QC and high-concentration QC samples (n = 3), spiked at mentioned concentrations. To examine the stability, QC hair samples were left for 10 days prior to sample preparation and analysis. The internal standard used was a deuterated 6-MAM. These samples were analyzed, and the peak area ratios were compared with the ones obtained by the analysis of freshly prepared samples.
Five different hair samples obtained from non-heroin users were examined in a selectivity test. There were no interfering peaks at the retention times of the target compound and IS. Representative chromatograms obtained from blank hair and spiked hair samples are shown in Fig. 3.
The linearity was tested in the concentration ranges of 0.1–25.0 ng/mg for samples in both groups. The resultant values displayed calibration parameters like slope, intercept, and coefficient of determination found from the calibration curves (n = 3). The R2 values for the linear regression were above 0.990 for under investigation compounds in both groups, indicating excellent fitting. The calculated limit of detection (LOD) values for the regular addict group and rehab groups were 0.0745 and 0.0311 ng/mg, respectively, while the lower limit of quantification (LLOQ) values were 0.22 and 0.09 ng/mg, respectively. The retention time of 6-MAM for the rehab group was 7.22 min whereas for the regular addicts’ group it was 7.23 min (Table 1).
Recovery, accuracy, and precision
The recovery, accuracy, and precision are summarized in Table 2. The analytical recoveries were determined at three concentration levels in three replicates. The mean recoveries (%) for the target compound ranged from 86.84 to 91.78% and from 87.81 to 93.62% for 6-MAM in addicts and rehab groups, respectively. The intra-day and inter-day precisions for addicts and rehab groups were 0.8 to 8.1% and 0.6 to 2.6% and 3.9 to 4.9% and 2.3 to 6.9%, respectively, whereas the intra-day and inter-day accuracies for both groups were ranged from −5.1 to 4.7% and −8.7 to −1.2% and −4.2 to 2.9% and −4.8 to 1.1%, respectively. These data were within the acceptance criteria of 15% of nominal concentration for low, middle, and high-quality control (QC) concentrations.
Carry-over and stability
To evaluate the absence of carry-over, the highest extracted calibrator was injected into the GC–MS instrument, followed by an ethyl acetate blank. Potential carry-over effects were not observed. For practical purposes, ethyl acetate blanks were used throughout the sample sequence to verify that no sample-to-sample contamination occurred. Stability experiments were performed to identify any possible variation due to storage time. Low- and high-concentration QC samples were analyzed using a method developed in this study. The results from the stability experiments indicate that the samples were stable under normal storage conditions. No significant loss of the target compound of 6-MAM was observed at room temperature for 10 days.
The random heroin addicts’ group had a mean age of 29.70 (SD 8.6, 17–46 years) and had mainly black hair color (75%) and mean duration of drug abuse of 3.3 years and median 6-MAM levels 7.815 ng/mg with SD 1.804, and the limit of detection was 0.1 ng/mg (Tables 3 and 4). The subjects from the rehab group had a mean age of 27.75 (SD 7.5, 17–46 years) and mean duration of heroin abuse of 4.8 years and median 6-MAM levels 2.3445 ng/mg with SD 1.548, and the limit of detection was 0.1 ng/mg  (Tables 5 and 6). The results suggest that the heroin addicts belonging to both groups had similar characteristics but there was a remarkable difference in mean 6-MAM levels between the two groups with lower mean 6-MAM levels in addicts undergoing rehabilitation at the de-addiction center group. This establishes a realistic efficiency of the de-addiction center as compared to the random addicts’ group.
Hair sample and 6-MAM concentrations
Interpretation of the mass spectrum for regular addicts’ sample
The key ions detected in this spectrum were of 6-monoacetylmorphine and morphine forming peaks at m/z 327 and 286, respectively. The mass-spectral fragmentation of these two key ions detected in this spectrum included a fragment forming a peak at m/z 229, formed due to the loss of CH2CHNCH3 from the original morphine compound. The loss of H2O and CH3CH=O from the fragment ion at m/z 229 results in the formation of fragment ions with peaks at m/z 211 and m/z 185, respectively. The loss of CO2 from the ion at m/z 185 results in the formation of fragment ion at m/z 157. The presence of morphine as well as 6-monoacetylmorphine along with the typical mass fragment pattern of morphine and other opiates (Fig. 1a, b) confirmed the abuse of heroin.
Interpretation of the mass spectrum of the sample from the de-addiction center group
The key ions detected in this spectrum were 6-monoacetylemorphine and morphine forming peaks at (m/z) 331 and 286, respectively. The mass-spectral fragmentation of morphine key ion detected in this spectrum included a fragment forming a peak at m/z 229, formed due to the loss of CH2CHNCH3 from the original morphine compound. The loss of CH3CH=O from the fragment ion at m/z 229 results in the formation of fragment ion with peaks at m/z 185. The loss of CO2 from the ion at m/z 185 results in the formation of fragment ion at m/z 157. The presence of morphine along with the typical mass fragment pattern of morphine (Fig. 2a, b) confirmed the prior abuse of heroin.
The data were first tested for normality by applying the Kolmogorov–Smirnov test and Shapiro–Wilk’s test. The Kolmogorov–Smirnov statistic (0.100, d.f 40, P 0.200) and Shapiro–Wilk’s statistic (0.943, d.f 40, P 0.042) combined signify that the data is not normally distributed. Furthermore, Levene’s statistics were applied to test for the difference of normality between the two groups. The results of Levene’s statistics (0.878, d.f 1,37.99, P 0.355) suggest that there is no significant difference in variance between the two groups. Leven’s statistics further justifies the application of the Mann–Whitney U test for comparison of the two groups since the data were not normally distributed. Table 7 shows the actual value of Mann–Whitney U statistics along with the P-value. From this data, it can be concluded that the 6-MAM levels in the random heroin addicts’ group are significantly higher as compared to subjects from the de-addiction center group (U = 0.000, P=0.000).
Figure 3 shows the mean ranks of the groups under study and suggests that the random heroin addicts’ group has a higher mean rank as compared to subjects undergoing rehabilitation at the de-addiction center. This implies that the random heroin addicts’ group has higher 6-MAM levels compared to subjects undergoing rehabilitation at the de-addiction center. The result showed that the de-addiction center offers remarkable success in lowering down the 6-MAM levels among the addicts undergoing a rehabilitation program.
Descriptive statistics were used to explain the characteristics of the heroin addicts belonging to two groups, i.e., random heroin addicts and the heroin addict subjects from the de-addiction center. The Kolmogorov–Smirnov test and Shapiro–Wilk test were applied to analyze the normality of the data. Furthermore, Levene’s test was applied to test homogeneity of variance prior to applying the Mann–Whitney U test to assure that there is no significant difference in variances of the two groups. Finally, since the data was not normally distributed, so the Mann–Whitney U test was applied to demonstrate the difference in 6-MAM levels between the two groups (Fig. 4).
The scope of hair analysis depends upon the detection of the drug and/or its metabolite, followed by its quantification in the hair matrix . A hair does not have its own metabolism or excretion system for flushing out the drugs that get incorporated into it . Hair analysis has an important place in forensic toxicology. In majority of cases, by the time drug-facilitated crimes were reported to the police, the drugs in question were frequently eliminated from the more conventional route, such as blood or urine, also keeping in mind the fact that hair does not decay as compared to other biological samples . In the current study, the quantity of 6-MAM in regular heroin addicts was found to be in a range of 5.46–10.7 ng/mg (mean value, 7.80 ng/mg) and that of subjects going through rehabilitation at the de-addiction center was in the range of 0.39–4.65ng/mg (mean value, 2.34 ng/mg), which is consistent with the prior findings led by Klug et al. and Marigo et al. in accordance with their findings [25, 26]. They had fulfilled the necessities for hair analysis by validating the RIA results by thin-layer chromatography (TLC) with fluorescence detection and using HPLC with fluorometric detection . Goldberger et al. identified heroin and 6-acetylemorphine for the first time in 1991 using GC–MS for the detection of drug in the hair sample . Kintz et al. in 1992 confirmed the existence of morphine in the hair sample ranging from 0.41 to 11.74 ng/mg, which is also consistent with the results of the present study . In the current study, we analyzed the level of 6-MAM in regular heroin addicts and in subjects undergoing rehabilitation at the de-addiction center. It is morphine and more recently 6-MAM, the chief metabolite of heroin, which are associated with significant consideration in the field of forensic science . The results obtained confirmed the presence of noticeable levels of 6-MAM in 100% of the samples, even 30 days after the abstinence of heroin. Therefore, on the basis of previous studies and results and findings of our study, it can therefore be concluded that in heroin addicts 6-MAM remains in hair samples even after 30 days of its abstinence. Moreover, it requires further study to precisely determine the transit time of the noticeable metabolite of heroin in the hair sample. In the future, more studies will need to be carried out to ascertain the timeframe window for the drug and/or its metabolites’ retention time in hair samples of definite heroin abusers.
It can be concluded that the method was successful in detecting the immediate metabolite of heroin, i.e., 6-monoacetylmorphine (6-MAM), in the hair samples of heroin abusers. This study undoubtedly advocates that once 6-MAM gets combined with hair follicles, it will remain at that place for more than 30 days after the last intake. However, in the future, the advancement of scientific tools and sophistication in analytical techniques will further facilitate the investigation of abused drugs at even lower limits with greater accuracy. These data also propose that hair analysis for 6-monoacetylmorphine can be used to discriminate heroin users from other types of opiate exposure (e.g., poppy seed, licit morphine, and codeine).
Availability of data and materials
Data and material are available upon request.
Certified Reference Material
Methyl tertiary butyl ether
High-pressure liquid chromatography
Gas chromatography–mass spectrometry
Selective ion monitoring
National Police Bureau
Food and Drug Administration
Office of Research, Innovation and Commercialization
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We are really thankful to the Pakistan Council of Scientific & Industrial Research Laboratories Complex (PCSIR) Pakistan for providing us the facilities to conduct research work. We are also thankful to laboratory staff for their invaluable support. We are highly thankful to the University laboratory staff who are always there to assist us even during the off hours.
Ethics approval and consent to participate
The study approval was given by the Department of Chemistry and Life Sciences, Government College University (GCU), Lahore, with approval letter number, GCU/1-056/CLS. The participants were included after approval from the “Office of Research, Innovation and Commercialization” (ORIC) with reference number 2017-0057. All the participants were informed about the study protocol and then included after thoroughly briefing about the research purpose as directed from the ORIC in the ethical guideline. The participants agree to co-operate for the said purpose by giving their written and verbal consent, which was in accordance with ORIC guidelines.
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Ghauri, M.A., Hassan, F., Hassan, Y. et al. Detection of 6-monoacetylemorphine in hair sample of heroin addicts using gas chromatography–mass spectrometry and significance of rehabilitation program. Futur J Pharm Sci 7, 98 (2021). https://doi.org/10.1186/s43094-021-00245-z