Table 1 Different trials
Trial | Column | Buffer | Mobile phase | Flow rate ml/min | Observation |
|---|---|---|---|---|---|
1 | Zorbax eclipse XDB-Phenyl (250×4.6mm, 5μm) | 0.1% (V/V) formic acid | Buffer: ACN (50:50 v/v) | 1.23 | Peak tailing (1.3) and long retention time (9.52 min) of EVZ |
2 | Zorbax SB C8 (100×4.6mm, 5μm) | 0.1% (V/V) formic acid | Buffer: ACN (35:65 v/v) | 1.23 | Resolution (1.5) was not good between LAM and TDF |
3 | Inertsil C8 (150×4.6mm, 5μm) | 0.1% (V/V) formic acid | Buffer: ACN (35:65 v/v) | 1.23 | Resolution (1.6) was not good between LAM and TDF |
4 | Zorbax eclipse XDB-phenyl (250×4.6mm, 5μm) | 0.1% (V/V) formic acid | Buffer: ACN (30:70 v/v) | 1.23 | Resolution was good, but retention time of EFV was long (8.056 min) |
5 | Phenyl XDB (250×4.6mm, 5μm) | 0.1% (V/V) formic acid | Buffer: methanol (50:50 v/v) | 1 | Long retention time of EFV (7.201 min) |
6 | Phenyl XDB (250×4.6mm, 5μm) | 0.1% (V/V) formic acid | Buffer: methanol (27:73 v/v) | 1 | Good resolution between LAM and TDF (9.13), TDF and EVZ (7.55), and less retention time (LAM-2.6 min, TDF-4.4 min, EVZ-5.9 min) |