Table 2 Optimization of parameters for analysis of ceftriaxone sodium using CCD
From: QbD approach to HPLC method development and validation of ceftriaxone sodium
Run | Factor-1 Acetonitrile to water | Factor-2 pH | Response-1 Retention time | Response-2 Theoretical plate | Response-3 Peak asymmetry |
|---|---|---|---|---|---|
1 | 70:30 | 6.5 | 4.033 | 5263 | 1.41 |
2 | 70:30 | 7.5 | 8.162 | 1798 | 1.66 |
3 | 75:25 | 5.5 | 3.468 | 1065 | 1.99 |
4 | 75:25 | 7.5 | 8.125 | 2352 | 1.99 |
5 | 65:35 | 6.5 | 4.017 | 8562 | 1.02 |
6 | 65:35 | 5.5 | 3.256 | 1523 | 1.96 |
7 | 75:25 | 6.5 | 4.018 | 5385 | 1.55 |
8 | 70:30 | 6.5 | 4.033 | 5263 | 1.41 |
9 | 70:30 | 5.5 | 3.265 | 2046 | 1.66 |
10 | 65:35 | 7.5 | 8.126 | 1689 | 1.85 |
11 | 70:30 | 6.5 | 4.033 | 5263 | 1.41 |