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Table 4 Results of different analytical method validation parameters

From: Formulation, optimization, qualitative and quantitative analysis of new dosage form of corticosteroid




The method was found selective and specific for triamcinolone acetonide eye drops (suspension), since there is no any interference from placebo in the retention time of triamcinolone acetonide active pharmaceutical drug


A linear relation was found for this HPLC method. The correlation co-efficient was found to be 1.00


The results were found within the acceptance value of relative standard deviation (% RSD ≤ 1.0%.)

System precision

% RSD of triamcinolone acetonide peak areas for six replicate injections of standard solutions are 0.395%, which is less than ≤ 1.0%

Intraday precision

Cumulative intraday precision of triamcinolone acetonide is 101.48% with cumulative RSD 0.22% respectively

Inter-day precision

Cumulative inter-day precision of triamcinolone acetonide 101.71% with cumulative RSD 0.31% respectively

Accuracy & Recovery

The amount of triamcinolone acetonide recovered in recovery I for eye drop were 101.68% with RSD 1.17% respectively


Range was found to cover 70–130% of the sample concentration

Stability of analytical test solution

The analytical solution is stable for 24 h when stored at room temperature assay value for 100.37% with RSD 0.87% respectively

LOD (µg/mL)

Triamcinolone acetonide (active drug) LOD: 0.096 (µg/mL)

LOQ (µg/mL)

Triamcinolone acetonide (active drug) LOQ: 0.31 (µg/mL)