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Formulation, optimization, qualitative and quantitative analysis of new dosage form of corticosteroid

Abstract

Background

The objective of the present research work is to develop the entirely new drug product formulation (Ophthalmic Suspension) which belongs to the therapeutic class of steroids drugs (Corticosteroid). There are different dosage forms of Triamcinolone acetonide to treat different systemic as well as topical diseases as per patient symptomatic conditions. Due to non-availability of this active drug (Triamcinolone acetonide) ophthalmic dosage form in the national and international market research work is carried out to enter in this dosage form.

Results

In the formulation design and development, different inactive like polysorbate 30 mg/mL, PEG-12 glyceryl dimyristate 70 mg/mL, ethyl alcohol 10 µL/mL, citric acid 1 mg/mL, sodium citrate 3.5–4.0 mg/mL, BKC 0.15 mg/mL and water for injection have been used at different stages in different proportions during the manufacturing procedure. Gradient HPLC, C18 column, 5 µm, 100 A, 3.9 mm × 300 mm, detector UV/Vis @ 254 nm, column oven, auto-sampler, degasser, is used and this analytical testing method is validated to obtain the accurate results. The excipients play different roles including solvent, antioxidant, solubilizer, emulsifier, antibacterial, preservative, anticoagulant, antimicrobial agent, surfactant and buffers in the stability of active drug for its excellent therapeutic output results. Correlation coefficient factor is greater than 0.999 which indicates that the method has good linearity at proposed concentration against triamcinolone acetonide.

Conclusions

Excellent physical and chemical properties like physical appearance, pH, specific gravity, viscosity, re-suspendability, sedimentation volume is accomplished to meet the requirements of the eye treatment products. On the basis of experimental results achieved during validation process, it can be assumed that the current analysis method is more accurate, precise, specific, linear and consistency indicating in the range of 70 to 130% of the analyte concentration. From the physical, chemical behaviors and chemical assay of the newly developed drug formulation of corticosteroid (triamcinolone acetonide) it is concluded that it can be probably put for further clinical trial in different phases for human use.

Background

The formulation development includes developing and manufacturing of desired products of active ingredients which are both stable and acceptable to the patient for required usage or need. Different formulations in development stages of different dosage forms required excipients of different properties like particle size, pH, solubility, diluent, preservative, binders, modifiers etc. Medicinal drug products are formulated to counter the disease, patient safety and confidence build up on the basis of consistency of quality attributes retained within the assigned specification of the drugs and drug products [1, 2].

Ophthalmic products are mostly developed for children, adults and elderly patients and poorly accepted by children. To avoid the use of preservatives with a possible harmfulness to the cornea or mucous membranes of an eye, single dose or multi-dose preparation in a special multi-dose container that does not require its contents to be preserved, should be considered for children. Chemically steroids are the organic natural compounds which are linked with each other in a specific way of four cycloalkane rings [3].

Two major types of steroids are existing within the human body. First corticosteroids found in adrenal gland present on kidney, aldosterone, which is controlling sodium concentration in the body. Second cortisol, which is controlling the body, such as serving as part of the body’s stress response system to decrease inflammation. Steroids are short form of corticosteroids, synthetic products look like cortisol, a hormone that adrenal glands produce naturally. Triamcinolone acetonide is a synthetic glucocorticoid and it is an agonist of the glucocorticoid receptor, which is about five times as strong as cortisol. It has very little mineralocorticoid properties [4].

Triamcinolone acetonide is used in a number of veterinary medicine as a component in external balms, creams or ointments and in external sprays for the control of itching of the skin in dogs and other animals. Triamcinolone acetonide is used in human medicines and available in the form of ointment, cream, lotion, aerosol (spray), inhaler, tablets and injections in different label claims for oral practice as well as for skin and in paste form for mouth use [5].

Triamcinolone acetonide is used topically to heal the crusting, scaling, inflammation, itching, redness, dryness of skin and for skin issues like psoriasis i.e., dermal problem in which red, scaly coverings appears on some areas of the body and eczema i.e., dry and itchy skin, sometimes develop red, scaly rashes. Triamcinolone acetonide is considered as 9-Fluoro-11β,16α,17,21-tetrahydroxypregna-1,4-diene-3,20-dione cyclic 16,17-acetal with acetone. It has chemical formula C24H31 FO6 and molecular weight 434.51. Figure 1 shows structural formula of triamcinolone acetonide.

Fig. 1
figure 1

Structural formula representing the framework of Triamcinolone acetonide

Corticosteroids are natural hormones which produced in adrenal cortex of humans and other mammals, in the same way analogues of these hormones. There are two types of these corticosteroids, one glucocorticoids and other mineralocorticoids, which are playing major role in a wide range of biological processes, together with stress, immune system and regulation of inflammation and metabolism response of the vertebrates. These corticosteroids bind with plasma proteins in variable ranges and are processed mostly in the liver and are then expelled by the kidneys [6].

Different dosage forms of triamcinolone acetonide include nasal sprays, Inhaler, Oral, Topical and Injectable corticosteroids. Triamcinolone acetonide injection in suspension form, is a non-natural glucocorticoid, having anti-inflammatory properties. This preparation is appropriate for intramuscular (IM) and intra-articular (IV) use only. Triamcinolone acetonide injection is used to address a number of swelling and other conditions, like severe allergic reactions, skin issues, severe colitis, swelling of the joints or tendons, blood cell disorders, lung disorders, inflammatory eye disorders and some problems associated with by low adrenal gland hormones. Triamcinolone acetonide is an extra powerful derived from triamcinolone, and is almost eight times much better than triamcinolone itself [7].

The present formulation development related to a kind of ophthalmic suspension preparation, be specifically related to a kind of ophthalmic drug product that contains triamcinolone acetonide for treating ophthalmic diseases.

Methods

Triamcinolone acetonide (API) and working standard got from a well-known national pharmaceutical unit. Non-active pharmaceutical ingredient (Excipients) were purchased from the commercial market of Lahore. Each of the excipient used in the preparation or design and development plays a significant role for the integrity of formulation of this ophthalmic drug product. These excipients are non-toxic, pharmacologically inert, physically & chemically stable, commercially available, acceptable to the regulatory point of view, have pleasing organoleptic properties and economical. Table 1 shows the role of non-active ingredients in formulation.

Table 1 Role of non-active pharmaceutical ingredient in formulation

For the identification of active drug material FTIR@ATR (Fig. 2) was performed and found comply with working standard given by the supplier.

Fig. 2
figure 2

FTIR spectrum of triamcinolone acetonide (Active drug)

Drug product master formulation

Master formulation of a drug product is a complete recipe of active drug and other ingredients in a specific quantity. It the master formulation of a drug product which makes it stable for long run use, any change in quantity and excipients makes it unstable. Drug product formulation is the process of mixing many chemical substances with the active drug to form a final medicinal product, which is called a medicine or drug product. Table 2 shows the drug product formulation.

Table 2 Master formulation of triamcinolone acetonide eye drops suspension

Manufacturing procedure of drug product

Pour the ‘’Polysorbate 80’’ (Tween 80), ‘’PEG-12 Glyceryl dimeristate’’ in a gradated glass beaker of 1000 mL and later 300 mL water for injection in it, mixed well to homogenize it with the help of a magnetic stirrer for 5 min with continuously stirring at 1000 rpm. Raised the mixture temperature up to 121 °C with continuous stirring and heated for 15 min with continuously stirring at 1000 rpm. Cool down the mixture with continuously stirring up to 50 to 60 °C. Added “Triamcinolone acetonide’’ which is active pharmaceutical ingredient and mixed continuously at 1000 rpm for 10 min. Cool down the total mixture with continuously stirring to 25–30 °C. Added up to 50% water for injection of the total volume of the product slowly slowly and mixed by continuously stirring for 5 min at 1000 rpm.

After checking the temperature 25–30 °C added the ‘’Ethyl Alcohol’’ in the above mixture and continuously at 800 rpm for 5 min. Prepared the buffer solution separately by adding one by one and continuously mixed for 5 min at 800 revolutions per minute at each addition in almost 10% of the total volume by adding Citric acid & Sodium citrate 2H2O and Benzalkonium chloride. Added filtered (0.2 micron) solution of separately prepared of Citric acid, Sodium citrate dihydrate and Benzalkonium chloride in 10% total volume of drug product in water for injection slowly. Mixed the above solution continuously for 10 min at 900 rpm for achieving the light milky suspension. Make up the final volume with the help of water for injection and mixed for 10 min at 900 rpm. White to off white colored drug product has been prepared, put the sample for analysis for physical and chemical parameters.

Chromatographic separation and resolution between the different components also depend on length, diameter, pore size, particle size of HPLC column, nature of mobile phase, pH and flow rate of mobile solution/phase. High performance liquid chromatography is a noteworthy practice for separation by adsorption mechanism. When the solute components passing through stationary phase’s porous material, the components interact with adsorption surface sites by competing with the eluent components for better adsorption sites.

After the manufacturing procedure, a product is obtained which contains the API (drug triamcinolone acetonide) and excipients in a specified amount which gives the stability to the product [8, 9]. Table 3 indicates the physicochemical properties of eye drop suspension.

Table 3 Physical and chemical properties of the eye drop suspension

HPLC method of analysis of triamcinolone acetonide eye drops suspension

Analytical method describes the parameters to be carried out for analysis of pharmaceutical drug product triamcinolone acetonide eye drops (Suspension) [10, 11]. Mix 65% HPLC water and 35% Acetonitrile and filter through 0.45 micron, sonicate and degas. Mix acetonitrile and water HPLC grade in ratio 40:60 and allow to temper before use.

Weigh accurately approximately 25 mg of working standard of Triamcinolone acetonide and shift in 25 mL vol. glass flask. Added 7 mL of analytical standard methanol and 3 mL acetonitrile, sonicate it in ultrasonic device for 10 min. Add 10 mL of diluent and leave it in ultrasonic bath until it dissolves. The volume is then made up to 25 mL. This solution contains 1 mg/mL of triamcinolone acetonide. Mix the sample (suspension) well with glass rod. Transferred a 1 mL aliquot part of Triamcinolone acetonide ophthalmic eye drop suspension, equivalent to 1 mg of Triamcinolone acetonide active pharmaceutical ingredient in 10 ml vol. glass flask. Added 7 ml of diluent solvent, then shake with ultrasound for 5 min. Then dilute with diluent solvent and mix. Centrifuge at 10,000 rpm for 5 min, separate supernatant liquid, filter supernatant through 0.45 µm membrane filter. This solution contains a theoretical concentration of 100 µg/mL of Triamcinolone Acetonide. Transfer to chromatograph vials.

As per International Council for Harmonization guideline (ICH) injected the reference standard solution triamcinolone acetonide six times under the described operating conditions. Record the chromatograms and verify that the following parameters are achieved: The coefficient of variation: NMT 2.0%, Coleus factor: NMT 2.0, The capacity factor: NLT 2.0 and relative Retention Times: 10–11 min.

Chemical assay procedure

Injected 20 µl of sample solution of triamcinolone acetonide suspension and standard solution of triamcinolone acetonide solution into the HPLC maintaining above chromatographic conditions, record the results in form of chromatograms and measure the responses of peaks.

The percentage of triamcinolone acetonide were calculated with reference to concentration of triamcinolone acetonide in the ophthalmic eye drops suspension using the following given formula.

$$\begin{aligned} & {\text{Chemical assay}} = \left( {{\text{rU}}/{\text{rS}}} \right) \times \left( {{\text{cS}}/{\text{cU}}} \right) \times {\text{P}} \\ & {\text{Response of sample solution\;(rU)}} = 3104.746 \\ & {\text{Response of Standard solution}}({\text{rS}}) = 3211.063 \\ & {\text{Concentration in the standard solution}}\;\left( {{\text{cS}}} \right) = 0.{1}0{3}\;{\text{mg}}/{\text{mL}} \\ & {\text{Concentration in the sample solution}}\;\left( {{\text{cU}}} \right) = 0.{1}\;{\text{mg}}/{\text{mL}} \\ & {\text{Purity of the working standard (P)}} = 99.94\% \\ & {\text{Chemical assay}}\; = 3104.746/3211.063 \times 0.103/0.1 \times 99.94 = 99.52\% \\ \end{aligned}$$

Validation procedure for triamcinolone acetonide eye drops

Triamcinolone acetonide standard solution

Weigh precisely 25 mg of working standard of triamcinolone acetonide and transfer in 25 mL vol. flask. Added 7 mL of HPLC grade methanol, 3 mL HPLC grade acetonitrile, sonicate it in ultrasonic 10 min. Now added 10 mL of diluent and leave it in ultrasonic bath until it dissolves. Temper it and dilute it with diluent to make up the volume. This solution contains 1.0 mg/mL of triamcinolone acetonide.

Determination of linearity and range

Transfer quantities of stock solution to a 25 mL volumetric flask respectively to prepare the required concentrations of triamcinolone acetonide as shown. Diluent used to make the volume and mix well.

Determination of precision (Intraday and Inter-day assay)

Determine the intra-day assay precision by analyzing samples repeatedly (3x) times in a day. Determine inter-day precision by analyzing the sample over 3 days and preferably by changing the analyst in same lab. On same HPLC instrument.

Determination of accuracy

Transfer 2 g triamcinolone acetonide, 30 g polysorbate (Tween 80), 70 g PEG-12, 10 mL ethyl alcohol, 1 g citric acid, 4 g sodium citrate dihydrate, 0.150 g benzalkonium chloride and water for injection (q.s) 1 L, mixing by stirring as in above preparation procedure to find a homogenous suspension. Take a quantity of sample suspension equivalent to 25 mg of triamcinolone acetonide in 25 mL vol. glass flask, added 7 mL of analytical grade methanol, 3 mL of analytical grade acetonitrile, sonicate it in ultrasonic 10 min. Added 10 mL of diluent and leave it in ultrasonic bath until it homogenizes. Warm it and dilute it with diluent to make up the volume. This solution contains 1.0 mg/mL of triamcinolone acetonide.

Now prepare the following strengths from the stock solution with diluent in 25 mL volumetric flask.

Weigh accurately 250 mg of triamcinolone acetonide working standard into a volumetric flask of 100 mL, added 28 mL of analytical grade methanol and 12 mL of analytical grade acetonitrile, sonicate it in ultrasonic for 10 min. Added 40 mL of diluent to dissolve and complete to volume with same diluent. Take 10 mL from stock solution ‘’A’’ in 25 mL vol. flask, make the capacity up to spot with diluent solution. Take 10 mL from stock solution ‘’A’’ in 50 mL vol. flask and make the capacity up to mark with diluent solution. Take 10 mL from stock solution ‘’A’ in 100 mL vol. glass and make the capacity up to mark with diluent solution. Now mix above stock solutions to get 1000 µg/mL, 500 µg/mL and 250 µg/mL of triamcinolone acetonide respectively as shown.

Placebo

Transfer 30 g Polysorbate (Tween 80), 70 g PEG-12, 10 mL Ethyl Alcohol, 1 g Citric Acid, 4 g Sodium Citrate Dihydrate, 0.150 g Benzalkonium Chloride and Water for Injection (q.s) 1 L, mixing by stirring as in above ‘’Preparation of Drug Product’’ to find a homogenous suspension. Proceeded as ‘’Sample Solution Preparation of Triamcinolone Acetonide Suspension’’ and then injected as a sample.

Results

The results are mentioned in Tables 4, 5, 6, 7, 8 and 9.

Table 4 Results of different analytical method validation parameters
Table 5 Chromatographic data of working standards and drug product
Table 6 System suitability data for T. acetonide eye drops suspension
Table 7 Triamcinolone acetonide range & linearity
Table 8 Intraday and inter-day precision for Triamcinolone acetonide
Table 9 Data showing the results for recovery I and II

Six repeated injections of standard solution (100% concentration) of triamcinolone acetonide were injected into the HPLC chromatograph and the results are shown. The %RSD for 6 injections of standard solution for triamcinolone acetonide was found to be 0.395%, indicating the results are within acceptable precision. Figure 3 represents chromatogram for standard injection.

Fig. 3
figure 3

Chromatogram showing the standard injection

Here RSD% is less than 1.0% for triamcinolone acetonide against 6 replicated injections as indicated above, so it was considered satisfactory for the evaluation of other performance parameters.

Determination of range and linearity

The linearity of triamcinolone acetonide (Fig. 4) was determined in the specified range. The results are presented graphically. The results indicate that the response for the triamcinolone acetonide peak was linear over the specified range 70–130% of the sample concentration.

Fig. 4
figure 4

Graph showing linearity for Triamcinolone acetonide

Determination of precision

Intraday & inter-day for Triamcinolone acetonide

The precision level of the triamcinolone acetonide eye drops suspension sample was found by replicate analysis. Cumulative intraday and inter-day variability were below 2% for triamcinolone acetonide as shown below. The intraday and inter-day accuracy of the triamcinolone acetonide was 101.02% and 101.08% with cumulative RSD 0.06% and 0.082% respectively. The results below show that the given method was accurate and precise.

Accuracy and recovery

Transfer a 1 mL aliquot part of triamcinolone acetonide ophthalmic eye drop suspension, equivalent to 1 mg of triamcinolone acetonide active pharmaceutical ingredient in a 10 mL vol. glass flask. Added 7 mL of diluent, and shake with ultrasound up to 5 min.

Temper and dilute with diluent and then mix. Centrifuge at 10,000 rpm for 5 min, separate supernatant liquid, filter supernatant through 0.45 µm membrane filter. This solution contains a theoretical concentration of 1000 µg/mL of triamcinolone acetonide. The mobile phase was used to dilute the stock solution to achieve 70–130% sample concentration. The amounts recovered were calculated by using following formula.

% Recovery = (Cfound/Cadded × 100).

The results indicate that the procedure has acceptable recovery level.

The mean recovery and relative standard deviation for standard solutions with excipients was found to be 101.6% with RSD 0.02% for Recovery I respectively. The excipients or inactive chemicals used in the drug product did not interfere in the analysis with active drug. The amount of T. acetonide recovered in Recovery II are 100.76% with RSD 0.53 respectively. The results indicate that the method has acceptable level of recovery.

Range

From the linearity, precision, accuracy as well as working range of the analytical method, was concluded to be 70–130% of the sample concentration.

Stability of standard solution

The sample solutions were prepared, analyzed initially at various time intervals and then stored at room temperature. On the basis of result, it is specified that the sample solution is stable for 24 h at room temperature.

Sensitivity (LOD &LOQ)

According to the ICH guidelines the LOD and LOQ can be determined by using the formula given: LOD was assessed as 3 × s/k while LOQ was assessed as 10 × s/k, here k represents, slope of the calibration curve while s represents the root mean square error regression standard deviation. Triamcinolone acetonide LOD value 0.096 µg/mL and LOQ value 0.31 µg/mL.

Discussion

The present drug product formulation development is an ophthalmic dosage form (Eye drops suspension) which belongs to the class of steroid. The active drug triamcinolone acetonide decreases chronic inflammation and other severe allergic reaction. Other dosage forms of the triamcinolone acetonide are present in the form of tablets, capsules, liquid injections, dry powder injections, creams/ointments, lotions, oral pastes, inhalers and nasal sprays in the market by manufacturer. The present ophthalmic formulation development was carried out to develop the eye drops suspension because of non-availability of this dosage form in the market on commercial scale on national as well as international level [12, 13].

Nowadays the suspension drug products formulations are also emerging in the form of eye drops of different drugs like the antibiotic and pain killers etc. In the development of triamcinolone acetonide eye drops suspension different inactive have been used as per drug product design and development which are inert itself and play different physiochemical roles in product formulation development, drug product characterization and stability for long term uses. The present developed formulation found stable as per different specifications [14].

Development of drug product

Each drug and drug product starts its finding and complete development in labs. Pharmaceutical organizations devote millions of rupees in such new investigations and development which comprises technical study and advancement of drugs for novelty. Capital investment may come from numerous areas as well as government grants, revenues, investors and donors etc. When the drug finding has been completed, its initial demand that it is essential to go for individually preclinical and clinical investigations with supportive results tangled to its assessment procedure. Preclinical investigation is a rudimentary initial stage that comprises checking of the drug on different animals and this initial checking is for protection measures [15,16,17].

This experimental (clinical) investigation is one of the utmost important step ladders in a drug’s growth or development. If any drug passes such initial stage experimental tests or trials, then it is shifted next to experimental (clinical) testing which includes human beings’ trials on volunteers. During new drug formulation advancement stages, the option of usage of new excipients and other materials depends on physiochemical behavior and active drug stability, like pH for an optical preparation is 7.4, comparable to eye tear liquid. However, most of active drug’s products in finished form are unstable at this pH, so it is too important to understand the active drug properties. Therefore, if buffers chemicals are involved, must enable pH as close as possible to the functional pH, and not creating any chemical instability.

When the preparation profile is recognized, the initial stage in product development is launching its physical and chemical characteristics like as physical appearance, thickness, viscosity, osmolarity and pH etc.

Mode of action of new development

The present drug product is ophthalmic dosage (Eye drops suspension) form which belongs to the class of steroid. Triamcinolone acetonide is the acetonide salt form of triamcinolone drug, a synthetic glucocorticosteroid with immunosuppressive and anti-inflammatory properties. Triamcinolone acetonide binds to a specific cytosolic glucocorticoid receptor and then interacts with glucocorticoid receptor response element on DNA and modifies gene expression. This results in an initiation of the synthesis of some anti-inflammatory proteins while inhibiting the synthesis of certain inflammatory mediators. Subsequently, a general decrease in chronic inflammation and autoimmune reactions are accomplished.

Other dosage forms of the triamcinolone acetonide are in the form of creams, ointments, lotions, oral pastes, inhalers, tablets, capsules, liquid injections, dry powder injections and nasal sprays in the market manufactured by different national and multinational pharmaceutical companies.

In the manufacturing procedure very economical and quite save methodology have been adopted to retain the efficacy of the parent drug (API). All the materials which have been used in the development are of pharmaceutical grade and tested properly before the development of the drug product to have the control and check on the properties of the drug product. Each excipient has its own specific properties to control the physicochemical properties to attain the particular specs in the drug product, similarly in the analytical work of the drug product all the chemicals which have been used are of high quality of world fame manufacturer with latest analytical equipment.

Conclusions

An ophthalmic product formulation may be in the form of a solution, suspension, an emulsion or ointment/cream. A representative eye attention or care drug product formulation must be sterilized, isosmotic, with some suitable pH property, comprises antimicrobial representatives with excellent container closure packaging, multi-dose or single dose administration system.

HPLC analysis method using reverse phase chromatographic technique with UV/Vis detector was established and validated for the determination of triamcinolone acetonide in a newly developed drug product at lab. scale for its intended use at commercial scale and consistency study analysis. The HPLC method specified in the procedure for qualitative and quantitative determination triamcinolone acetonide was confirmed for accuracy, precision, specificity, LOD, LOQ, linearity, stability of standard solution. This established HPLC method is rapid, economical and reliable for intended use and for the determination of a drug component in new developed and formulated ophthalmic dosage form triamcinolone acetonide eye drops suspension. The method designed specifically hasn’t any interference with excipients.

The significant feature of the analytical analysis method validation is that it was designed and assured gradient procedure which is easy to run on any kind or brand of HPLC. It is time saving, environmentally friendly. The current designed analysis procedure has reliably accurate and precise. It is concluded that this analytical method is valid for the identification and chemical assay determination of active ingredient (triamcinolone acetonide) in the newly developed ophthalmic dosage form.

Abbreviations

USP:

United States Pharmacopeia

NF:

National Formulary

CPS:

Counts per seconds

LOD:

Limit of Detection

LOQ:

Limit of Quantification

RSD:

Relative Standard Deviation

BP:

British Pharmacopeia

Ph. Eur:

European Pharmacopoeia

HPLC:

High performance liquid chromatography

ICH:

International Council for Harmonization

NMT:

Not more than

NLT:

Not Less than

BKC:

Benzalkonium chloride

PEG:

Poly Ethylene Glycol

IM:

Intramuscular

IV:

Intravenous

q.s:

Quantum satis

FTIR@ATR:

Fourier Transform Infra-Red with Attenuated Total Reflectance

Qty:

Quantity

COPD:

Chronic obstructive pulmonary disease

UV/Vis:

Ultraviolet-visible (UV-Vis) spectrophotometry

TAA:

Triamcinolone acetonide

RH:

Relative humidity

API:

Active pharmaceutical ingredient

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Acknowledgements

We are thankful for all those who have supported us during this project.

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AN contributed to the conception, design, interpretation of data and preparation of first draft. MM has performed all the experiments and was responsible for data acquisition. MI has proof read the draft of the work for the project and also helped in interpretation of data. All authors read and approved the final manuscript.

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Correspondence to Arif Nazir.

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Nazir, A., Masih, M. & Iqbal, M. Formulation, optimization, qualitative and quantitative analysis of new dosage form of corticosteroid. Futur J Pharm Sci 7, 208 (2021). https://doi.org/10.1186/s43094-021-00364-7

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  • DOI: https://doi.org/10.1186/s43094-021-00364-7

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