Table 1 Parameters for fingerprinting analysis
Parameters | Values |
|---|---|
Calibration parameters | |
Calibration mode | Single level |
Statistics mode | CV |
Evaluation mode | Peak height and area |
Linomat 5 application parameters | |
Spray gas | Inert gas |
Sample solvent type | Methanol |
Dosage speed | 150 nl/s |
Pre-dosage volume | 0.2 µl |
Syringe size | 100 µl |
Application position | 8.0 mm |
Band length | 6.0 mm |
Solvent front position | 85.0 mm |
Detection- camag TLC SCANNER | |
Position of Track | 10.0 mm |
Number of Track | 2 |
Distance between tracks | 10.0 mm |
Scan start position Y | 5.0 mm |
Scan end position Y | 75.0 mm |
Slit dimensions | 4X0.3 mm, Micro |
Optimize optical system | Light |
Scanning speed | 20 mm/s |
Data resolution | 100 µm/step |
Integration properties | |
Data filtering | Savitsky-Golay 7 |
Baseline correction | Lowest slope |
Peak threshold min. slope | 5 |
Peak threshold min. height | 10AU |
Peak threshold min. area | 50 |
Peak threshold max. height | 990 AU |
Track start position | 5.0 mm |
Track end position | 75.0 mm |
Display scaling | Automatic |
Measurement | |
Wavelength | 254 nm |
Lamp | D2& W |
Measurement type | Remission |
Measurement mode | Absorption |
Optical filter | Second order |
Detection mode | Automatic |
PM high voltage | 294 V |