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Elucidation of possible mechanisms of the antidiabetic potential of Zn-loaded Bryophyllum pinnatum (Lam.) extracts in experimental animal models
Future Journal of Pharmaceutical Sciences volume 8, Article number: 42 (2022)
The present study focused on the antidiabetic potential and fingerprinting analysis of Bryophyllumc pinnatum stems as well as its possible mechanisms of action along with identification of its major phytoconstituents. The oral glucose tolerance test has been performed administering a glucose solution (2000 mg/kg) to induce hyperglycemia. Diabetes have been instigated by single intra-peritoneal injection of streptozotocin (65 mg/kg).
Alcohol and aqueous extracts were found to be safe up to a dose of 3000 mg/kg. Oral glucose tolerance test results showed significant reduction in fasting blood glucose level. Alcohol and aqueous extracts (200, 400 mg/kg b.w.) showed significant reduction in fasting blood glucose among all groups. Groups receiving zinc sulfate-loaded extracts showed a statistically significant reduction in low-density lipoproteins, triglycerides and total cholesterol levels and enhanced levels of high density lipoproteins. Fingerprinting analysis has been performed to identify the major phytoconstituents of flavonoid category morin, chrysin, and 6-hydroxy flavones, as well as iso-quercetin, hyperosides and terpenoids present in the extracts possess antidiabetic potential.
Both alcohol and aqueous extracts found to possess significant antidiabetic activity in diabetic rats. Zinc sulfate synergistically potentiates the antidiabetic potential of alcohol extract. Fingerprinting analysis revealed the presence of flavonoids such as morin, chrysin, and 6-hydroxy flavones, as well as iso-quercetin, hyperosides, and terpenoids. The possible mechanisms of antidiabetic activity have been elucidated, although further studies are required to give more elaborated mechanism on molecular basis.
Bryophyllum pinnatum, also known as Air Plant, Life Plant, Miracle Leaf, Goethe Plant, Katakataka, Zakhmehaiya, and Astibhaksha, is a miracle plant that belongs to the Crassulaceae family . This plant can attain height of 1.5 m with hollow branching growth and can reach a height of 1–1.5 m and produces many bell-shaped blooms as shown in Fig. 1.
It is also known as the “wonder plant,” because leaf itself can give rise to a new plant. This plant possesses number of therapeutic properties like insect bites, ulcers, wounds, inflammations and discolorations, rheumatic infections and boils, cancer, viral, smallpox, diarrhea, dysentery, lithiasis and phthisis, conjunctivitis, earache, and sore throats are all treated with this plant (Fig. 2) [2, 3].
This plant has been widely studied for its phytoconstituents due to its immense therapeutic qualities. The primary chemical constituents discovered in B. pinnatum are 3,4-dimethoxy quercetin (1) (Darmawan et al. 2013), 4′,5-dihydroxy-3′,8-dimethoxyflavone 7-O-D-glucopyradinoside, and 4′,5-dihydroxy-3′,8-dimethoxyflavone 7-O-D-glucopyradinoside (2), bryophollenone (3), bersaldegenin (4), bryophynol (5), bryophollone (6) , Bryophyllol (7,8), bryophyllin (9,12), etc. (Fig. 3) [5, 6].
The plant has also been reported as a rich source of minerals, particularly Zn, vital for maintaining one's health. Plant leaves and roots are reported to possess antidiabetic potential . However, the stem extracts, have not yet been explored for any antidiabetic potential in STZ-induced type 2 diabetic rats. As a consequence, research into the antidiabetic potential of B. pinnatum stem extracts along with identification of its active phytoconstituents has been performed in the present work.
Sigma-Aldrich Co. (St. Louis, USA) provided the streptozotocin (STZ). Chemsynth laboratories Pvt. Ltd. (Haldwani, Uttarakhand, India) provided the solvents. ZnSO4 has been purchased from Eisen-Golden brand in powder form of reagent grade. Span Diagnostic Ltd in India provided the analytical grade chemicals and biochemical kits.
Stems of B. pinnatum were obtained from the herbal garden of Devsthali Vidyapeeth College of Pharmacy Rudrapur (Uttarakhand, India) in September. NBRI (Lucknow) has identified and authenticated the plant 2021. Herbarium specimen no. Varsha 030 was submitted at the Institute Museum.
Preparation of extracts
Fresh stems were cleaned thrice with water and dried at temperatures below 45 °C in a hot air oven. The BPSAL was prepared in a Soxhlet apparatus with 70% (v/v) alcohol for 48 h of continuous soxhlation followed by filtration and concentration. The mark has been dried before being transferred to a conical flask. BPSAQ has been prepared by using distilled water/CHCl3 (9:1) by cold maceration (Fig. 4) .
Transverse sections of the stem were used to identify the characteristics of the plant and the stem .
Physiochemical analysis has been performed as per reference .
Preliminary phytochemical analysis (PPA)
Both extracts were submitted to PPA to identify their major phytoconstituents, and then a series of TLC assays have been undertaken to identify the best suitable mobile phase for further chromatographic analysis [9, 10].
Thin layer chromatography has been performed by using four different mobile phases in order to identify the major phytoconstituents:
Mobile Phase I: Ethyl acetate: Formic acid: Glacial acetic acid: water [10:1.1:1.1:2.6].
Mobile Phase II: Ethyl acetate: Methanol: Ethanol: Water [8.1:1.1: 0.4:0].
Mobile Phase III: Ethyl acetate: Butanone: Formic acid: Water [5:3:1:1].
Mobile Phase IV: Iso-butanol: Acetic acid: Water [14:1:3.5].
BPSAL, BPSAQ have been then subjected to fingerprinting analysis in order to identify their key phytoconstituents .
Male wistar rats weighing 150–180 g were procured from the IVRI in Bareilly (Uttar Pradesh, India). The animals were raised under ideal conditions of temperature (25 °C), humidity (55–65%), and a 12-h light–dark cycle. The animals had unlimited access to food and water. Prior to the commencement of the experiments, the animals were familiarized with the lab conditions. The IAEC gave their approval to all of the tests. (CPCSEA/IAEC/2021-11/06).
Analysis of acute toxicity
The acute toxicity of the stem extracts has been performed as per OECD-420 guidelines. On healthyswiss albino mice (68 weeks) of either sex weighing 20–25 g were selected for toxicity analyses. Animal house was well maintained under standard hygienic conditions, at temperature (22 ± 2 °C), humidity (60% ± 10%) with 12 h day and night cycle, with food and water ad libitum. All pharmacological studies were carried out after obtaining approval from the Institutional Animal Ethics Committee of Devsthali Vidyapeeth College of Pharmacy (Rudrapur, Uttarakhand, India). The BPSAL, BPSAQ extracts were administered orally at doses of 5, 50, 300, 2000, 3000 mg/kg b.w. to the overnight fasted albino mice. Five animals were used for each dose and have been observed closely for the first 3 h, for any toxic manifestation (for example, increased motor activity, sedation, acute convulsion, coma and death). Thereafter, the observations were made at regular intervals for 24 h. The animals were under observation for 14 days [12, 13].
Oral glucose tolerance test (OGTT)
Wistar rats have been divided into six groups, each with six animals, after being fasted overnight.
Group 1 was given 5% tween 80,
Group 2 was given Glibenclamide (10 mg/kg),
Groups 3 and 4 were given ethanol extracts at 200, 400 mg/kg bw.
Groups 5 and 6 were given aqueous extracts at 200, 400 mg/kg bw.
Groups 3, 4, 5, and 6 were administered glucose solution (2000 mg/kg) orally 30 min after extract administration. Blood was collected from the retro orbital sinus puncture before and after administration of the extracts at regular intervals of 30 min at 0, 30, 60, 90, and 120 min after glucose administration. Fasting blood glucose (FBG) has been determined using a glucose detection kit (Span diagnostic pvt. Ltd, Surat, India). [13,14,15].
Induction of diabetes
A single i.p injection of a freshly prepared STZ (65 mg/kg) solution in buffer was used to induce diabetes (pH 4.5, citrate 0.1 M buffer). Blood samples were obtained from the retro orbital plexus 48 h after STZ injection, and plasma was separated from blood by centrifugation. FBG were tested using a GOD/POD kit to confirm diabetes.
Animals with diabetes were divided into 9 groups with six animals each.
Group 1: was provided with water and had unrestricted access to food and drink.
Group 2: The diabetic control group got 0.5 mL of 5% tween 80.
Group 3: Glibenclamide (10 mg/kg b.w.)
Groups 4,5: BPSAQ (200, 400 mg/kg b.w.)
Groups 6,7: BPSAL (200, 400 mg/kg b.w.)
Groups 8,9: BPSAL (200, 400 mg/kg b.w.) + ZnSO4 (10 mg/dl).
The experiment has been performed up to 21 days. Blood samples were taken from overnight-fasting rats (retro-orbital sinus) at 0, 7, 14, and 21 days of the experiment before drug administration. On day 0 and day 21, the body weight of the rats was measured. On day 0 and day 21, biochemical tests were performed to evaluate the plasma lipid profile (Fig. 5) .
The data are presented in the form of a mean with standard deviation. For statistical analysis, two-way ANOVA and DMRT (Duncan's multiple range tests) were employed. The findings were deemed statistically significant when the value was P < 0.05 or below.
Win-CATS4 software was used to run a Camag HPTLC system, Linomat V applicator, TLC scanner 3, Reprostar 3, and a 12-bit CCD camera for all of the images. Solvents for HPTLC were acquired from MERCK. All weighing was done with a computerized balance (Precisa XB 12A).
Samples were prepared by dissolving the extracts in appropriate solutions subjected to sonication and centrifugation. A sample of the supernatant solution has been taken for analysis.
The samples were prepared using the above-mentioned method. For 30 min, the pre-coated plates were activated in a hot air oven at 120 °C. Spots of BPSAL and BPSAQ (2 µl) each have been placed on pre-coated (silica gel-60 F254) plates with Linomat V applicator and Hamilton syringe triplicate (tracks 16). The chamber was saturated for at least 18 h, pre-coated plates with spots of BPSAL and BPSAQ were placed in chamber and allow to run up to a distance of 77 mm. The band length (8 mm), slit size, and scanning speed at 254 nm and 366 nm were scanned after the pre-coated plates were dried. Deuterium, tungsten, and mercury radiation sources were used for scanning of pre-coated plates at wavelengths of 254 nm, 366 nm, and 425 nm. Tables 1, 2 include fingerprinting analysis specifications. A software was used to calculate the retention factor (Rf) and peak area. Table 1 shows the parameters used in fingerprinting analysis and Table 2 shows different tracks representing sample position and volume [7, 14, 16].
The macroscopical characteristics of the plant sample received from a nursery were investigated. The stem displayed dark green hue, a distinct odor, a slightly bitter-pungent taste, and varied in size. On the outside, the stem has a thick cuticle and longitudinal lines/scars.
(a) Transverse section
Uniseriate trichomes with thick epidermis were visible in a transverse section. A ring of vascular bundle patches, primary xylem, phloem, and very little pith area have been found just beneath the large cortical region. Small circular starch grains in a chain, representing slight secondary growth were observable. As shown in Fig. 6, the presence of starch granules in a specific characteristic pattern in the cortical area and a distinct pattern of phloem at the border of the vascular bundle ring were diagnostic characteristics of the plant.
(b) Histochemical tests
Sections when treated with different reagents like phloroglucinol, ruthenium red, iodine, sudan red III and conc. HCl showed the presence of lignin, mucilage, starch, fixed oil and crystals (Table 3).
Parameters such as LOD, moisture content, Total ash, acid insoluble, water-soluble ash value, and extractive values (alcohol and water) of powdered drug were found to be 0.79, 12.5%, 9.58%, 2.45%, 5.68%, 8.94 and 12.64, respectively, as shown in Tables 4, 5 and 6.
Phytochemical analysis of Pet. Ether (60–80 °C), benzene, chloroform, acetone, alcohol and aqueous extracts has been performed. Phytosterols, fixed oils and fats were found to be present all extracts except acetone extracts. Alcohol extract was found to be rich in carbohydrates and glycosides, alkaloids, phenolic compounds and tannins, saponins and flavonoids. Aqueous extract was found to be rich in glycosides, phenolic compounds, flavonoids and saponins as shown in Table 7.
Fingerprinting analysis of BPSAL extracts (2 µl) revealed 11 major peaks (Rf 0.07, 0.14, 0.15, 0.20, 0.25, 0.28, 0.44, 0.51, 0.63, 0.75, 0.81) when run in mobile Phase I; five sharp peaks (Rf 0.47, 0.60, 0.75, 0.77, 0.82) when run in mobile phase II (Cardiac glycosides); Six major peaks (Rf 0.02,0.05,0.14,0.35,0.36,0.61) in mobile phase III; five prominent peaks (Rf 0.06, 0.16, 0.28, 0.42, 0.72) in mobile phase IV in Tables 8, 9, 10 and 11, Figs. 7 and 8A,C.
Among the above major peaks, peak at Rf 0.42 was identified to 7-hydroxy flavone, peaks at Rf at 0.14, 0.61 were identified as morin and hydroxy flavone, respectively, when compared with standard Rf . Spots at Rf 0.25 and 0.81 were identified as terpenoids (I & II), respectively, in BPSAL .
Fingerprinting analysis of BPSAQ (2 µl) revealed 8 prominent peaks (Rf 0.07, 0.11, 0.28, 0.44, 0.57, 0.62, 0.64, 0.71) when run in mobile phase I; 2 prominent peaks (Rf 0.01, 0.77) when run in mobile phase II; 4 major peaks (Rf 0.01, 0.09, 0.60,0.68) when run in mobile phase III; 3 sharp peaks at Rf 0.06, 0.17, 0.73 in mobile phase IV, respectively, as shown in Tables 12, 13, 14 and 15, Fig. 7 and 8B, D. Among above-mentioned Rf, peak at Rf 0.60 shows presence of Chrysin in aqueous extract when compared with standard Rf . Spots corresponding to Rf 0.77 and 0.68 showed the presence of Iso-quercetin and hyperosides, respectively, in aqueous extract when compared with standard Rf .
Fingerprinting analysis results showed that aqueous extract is rich in flavonoids and phenol constituents. BPSAQ showed major spots at Rf 0.09, 0.17, 0.23, 0.60, 0.68, 0.73, 0.77, 0.86 quenched fluorescence at 254 nm and 366 nm, respectively. All spots quenched fluorescence at 254 nm and showed blue and violet fluorescence at 366 nm. Figure 9 shows the three-dimensional view of extracts in all the four mobile phases. BPSAL showed 6 peaks, whereas BPSAQ showed 4 peaks. Peaks with Rf 0.14 and 0.61 in BPSAL and 0.60 in BPSAQ corresponds to morin, 6-hydroxy flavone and chrysin, respectively .
Acute toxicity studies
Toxicity analysis of extracts has been found to be safe up to dose of 3000 mg/kg b.w.
OGTT of BPSAL and BPSAQ extracts
Oral glucose tolerance analysis of BPSAL and BPSAQ is shown in Fig. 10. BPSAL and BPSAQ (200 and 400 mg/kg b. w) have not significantly reduced the FBG in OGTT analysis. This means the extracts do not exert significant effects on insulin secretion.
Anti-diabetic analysis results of BPSAL, BPSAQ are shown in Fig. 11. Both the extracts at 200, 400 mg/kg b.w. have significantly reduced the elevated FBG in diabetic rats. When compared among the extracts, BPSAQ at 400 mg/kg b.w. has shown maximum antidiabetic activity. BPSAL (200, 400 mg/kg b.w.) revealed the statistically significant activity (p > 0.05) when combined with ZnSO4 synergistically showed the marked decrease in FBG (p > 0.001) in STZ-induced diabetic studies. ZnSO4 synergistically augmented the antidiabetic potential of BPSAL comparable with that of standard drug. BPSAL + ZnSO4 (200,400 mg/kgbw) showed statistically significant reduction in LDL, TG and TC levels and elevated level of HDL when compared among all extracts (Fig. 12). BPASAL and BPSAQ does not show any weight gain, but ZnSO4 also showed synergistic effect on weight (Fig. 13).
Macroscopical studies showed dark green stem, distinct odor, a slightly bitter-pungent taste, and varied in size. On the outside, the stem has a thick cuticle and longitudinal lines/scars. Microscopical studies showed presence pf the thick cuticle and longitudinal lines/scars, Uniseriate trichomes with thick epidermis, ring of vascular bundle patches, primary xylem, phloem, and very little pith area have been found just beneath the large cortical region. Physiochemical analysis standardization of plant will be helpful in correct identification of plant in powdered form. Ash value, extractive value, histochemical analysis and microscopical analysis will be helpful in identification of correct species as well as variety.
Phytochemical analysis of Pet. Ether (60–80 °C), benzene, chloroform, acetone, alcohol and aqueous extracts have been performed. Phytosterols, fixed oils and fats were found to be present all extracts except acetone extracts. Alcohol extract was found to be rich in carbohydrates and glycosides, alkaloids, phenolic compounds and tannins, saponins and flavonoids. Aqueous extract was found to be rich in glycosides, phenolic compounds, flavonoids and saponins. Major phytoconstituents detected in fingerprinting analysis are morin, chrysin, 6-hydroxy flavones, iso-quercetin and hyperosides from flavonoid category and terpenoids. These flavonoids are reported to possess significant antidiabetic potential. Morin improves fructose-induced hepatic SphK1/S1P signaling pathway impairment, leads to reduction of hepatic NF-κB activation with IL-1b, IL-6 and TNF levels. Moreover, morin also reduces the lipid accumulation and reduces hyperlipidemia and recovered insulin and leptin sensitivity (Fig. 14).
Quercetin has been reported to act through many cellular mechanisms increasing SOD, GPX, CAT and inhibition of PI3K and GLUT2 receptors, blockade of tyrosine kinase, stimulation of GLUT4 in skeletal muscles and also act through AMPK activation. Isoflavones and phenolic phytoconstituents also play significant role to maintain glucose homeostasis through inhibition of tyrosine kinase enzyme and promoting cAMP/PKA signaling pathway .
Both the extracts were found to be safe up to the doses of 3000 mg/kg bw. Oral glucose tolerance analysis of BPSAL and BPSAQ (200 and 400 mg/kg b. w) have not significantly reduced the FBG in OGTT analysis. This indicates that extracts do not exert significant effects on insulin secretion. Antidiabetic potential of BPSAL, BPSAL + Zn and BPSAQ has been evaluated by STZ-induced models. BPSAL and BPSAQ does not show significant effect in reducing the FBG in OGTT analysis but showed significant reduction in FBG, LDL, TG and TC in STZ-induced diabetic analysis revealed its effects similar to Biguanides, which don’t enhance the insulin secretion but facilitate glucose absorption, reduces the glucose release from liver, better be called as “antihyperglycemic” rather than “hypoglycemic”.
The results indicates that extracts may acts as increasing the insulin sensitivity by increasing the glucose uptake through cells by various mechanisms similar to biguanides. Possible mechanism behind the observed significant antidiabetic potential lies in AMPK activation leads to suppression of anabolism and stimulation of catabolism leads to phosphorylation of cAMP specific 3′,5′-cyclic PDE4B and activates cAMP degradation. Consequently, it prevents the activation of cAMP-dependent PKA which further activates CREB-CBP-CRTC2 (CREB: CRTC2) transcription complex, PEPCK and G6Pase. AMPK also inhibits SREBP-1 which lead to reduction of FAS and SCD1 and ME and therefore inhibits fatty acid synthesis. Zn activates the AMPK through MKKβ, imparts synergistic role and further potentiates the antidiabetic potential of extracts [22, 23]. Figure 15.
Among all the extract BPSAL + ZnSO4 revealed most significant effect and reduces the LDL, TG and TC level and comparable with that of standard drug. Zn is essential for adequate function of most of the hormones, there are two prominent mechanism of action that may explain the significant antidiabetic action of BPSAL + ZnSO4. Zn interacts with insulin in three ways, i.e., (a) it binds with insulin in pancreas and stops its entry in blood, whenever not required, (b) it enhances the glucose absorption through by enhancing the binding of insulin to cells and fastens the glucose uptake inside the cells correctly known as “insulin sensitivity”, and (c) zinc showed marked anti-inflammatory potential degrade inflammatory markers (C-reactive proteins reduces the edema in cells and retains cell health as well as insulin sensitivity).
STZ destroys β-pancreatic cells via alkylation of DNA, entering through GLUT2 receptors, which in turn damages DNA through inducing ADP-ribosylation turns into NAD+ depletion, ATP within the cell results in diabetes. Besides, it also generates H2O2, OH radicals and toxic chemicals like nitric oxide that results in DNA damage in β-pancreatic cells.
Role of oxidative stress cannot be denied in diabetes; reactive oxygen species such as OH−, O2−, H2O2, NO− produced during metabolism may degrade protein and enzymes, plasmids, inhibiting protein synthesis, plasma membrane disruption and DNA degradation. ROS may hamper mitochondrial functions and trigger apoptotic pathways and also facilitate cell lysis through necrosis and apoptosis. ROS excessive production leads to oxidative DNA damage/mitotic death may also trigger autophagic/mitophagic cell death. Zn is required for synthesis of superoxide dismutase enzymes which in turn reduces the oxidative stress (Fig. 15). Besides it, Zn has also been reported to possess numerous therapeutic potentials such as anti-inflammatory, photocatalytic, wound healing, anticancer, antioxidant, antifungal, antibacterial activities, etc. .
Flavonoids, polyphenols, glycosides, steroids, tri-terpenoids, and phytosterols were found in BPSAQ and BPSAL, suggesting that they may help with antidiabetic activity [25, 26]. The presence of Zn in the extract could explain the substantial antihyperglycemic effect. It has previously been noted that this plant contains Zn, and some studies have shown that Zn supplementation can help diabetic patients control their blood glucose levels [27, 28].
Significant weight loss is a hallmark of STZ-induced diabetes, which was also seen in the present study. The BPSAL (400 mg/kg) and glibenclamide treated groups exhibited a substantial improvement in body weight (p < 0.05) when compared to the normal control group, but other extracts did not fully restore the weight loss. BPSAL + ZnSO4 showed statistically significant effects on body weight showed synergistic effect of Zn when combined with BPSAL.
The mechanism of action of Zn may involve binding with IGF-I, which facilitates diverse cell events like amino acid stimulation, glucose uptake, and cell cycle regulation; it also associates with a membrane-associated receptor with tyrosine kinase activity. When IGF-I activates the receptor, a sequence of phosphorylation takes place within the cell, mediating cellular functions. Insulin-like growth factor-I binds to IGF binding proteins in the circulation; at least eight distinct binding proteins have thus far been recognized. There is mounting evidence that IGF-I binding proteins are not inert carrier proteins, but rather part of a complicated system that regulates IGF-I availability to cells. Nutritional status has a strong influence on IGF-I levels in the blood. In particular, decreased serum IGF-I was observed in humans and animals in case of Zn deficiency (Fig. 16).
Therefore, ZnSO4 showed synergistic effect on antidiabetic potential of BPSAL that may act through various mechanism. Further studies are required to elucidate more specific mechanism of action of antidiabetic potential of extract with ZnSO4 .
The current study investigates the acute toxicity, antidiabetic, fingerprinting, and pharmacognostical evaluation of the B. pinnatum stem. Fingerprinting analysis reveals the presence of morin, chrysin, 6-hydroxy flavones, iso-quercetin and hyperosides flavonoid category and terpenoids are reported to possess significant antioxidant and antidiabetic potential through various mechanism such as SphK1/S1P signaling pathway impairment, leads to reduction of hepatic NF-κB activation, TNF-α, increasing SOD, GPX, CAT and inhibition of PI3K and GLUT2 receptors, blockade of tyrosine kinase, stimulation of GLUT4 in skeletal muscles and also act through AMPK activation (Fig. 16). Flavanoids such as quercetin, isoflavones, etc., may act through AMPK activation and stimulation of catabolism leads to phosphorylation of cAMP specific 3′,5′-cyclic PDE4B and activates cAMP breakdown. Subsequently, hampers cAMP-dependent PKA activation leads to activation of CREB-CBP-CRTC2 (CREB: CRTC2) transcription complex, PEPCK and G6Pase. Activated AMPK inhibits TORC2 and HNF4α which leads to effect of G6PC and PEPCK results in gluconeogenesis stimulation and reduces the blood glucose level. Zn activates the AMPK through MKKβ imparting synergistic role and further potentiates the antidiabetic potential of extracts.
ROS such as OH−, O2−, H2O2, NO− generated in metabolism may degrade protein and enzymes, plasmids, inhibits protein synthesis, plasma membrane disruption and DNA degradation. Zn is required for synthesis of Superoxide dismutase enzymes in turn reduces the oxidative stress. The mechanism of action of Zn may also involve in binding with IGF-I, which facilitates diverse cell events like amino acid stimulation, glucose uptake, and cell cycle regulation; it also associates with a membrane-associated receptor with tyrosine kinase activity. In acute toxicity testing, both extracts (BPSAL and BPSAQ) were determined to be safe, even at a high dose of 3000 mg/kg b.w. Both the extracts did not show significant potential in OGTT analysis but showed significant antidiabetic potential in STZ-induced diabetic evaluation. The antidiabetic potential was further enhanced when combined with ZnSO4.
The possible mechanism of action has been proposed but further studies are required to elaborate the exact mechanism of action at molecular level. The extract contains morin, chrysin and 6-hydroxy flavones, as well as iso-quercetin, terpenoids 1, 2, and hyperosides as confirmed in fingerprinting analysis. The antihyperglycemic action may be due to secondary metabolites in conjunction with minerals. More study is required to identify the precise mechanism of action and phytoconstituents responsible for the antihyperglycemic effect.
Availability of data and materials
All data and materials are available upon request.
- B. pinnata :
Oral glucose tolerance test
Blood plasma glucose level
Loss on drying
Fasting blood glucose
Protein kinase A
Activated protein kinase
Liver kinase B1
- Lep R:
- PP 2A:
Protein phosphatase 2A
Sterol regulatory element-binding proteins
HMG-CoA reductase = 3-hydroxy-3-methyl-glutaryl-CoA reductase
Carnitine palmitoyl-transferase I
Mammalian target of rapamycin
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The authors are grateful to the NBRI, Lucknow, India, for providing the necessary resources. Prof. Edwin Jarald of the B.R.N.C.O.P., Mandsaur, India, provided HPTLC chromatograms, and the management of Devsthali Vidyapeeth College of Pharmacy in Lalpur, Rudrapur, India, provided critical facilities.
This plant is abundantly available in India, which has been collected and processed as per national guidelines.
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Tiwari, V., Tiwari, A., Singh, A. et al. Elucidation of possible mechanisms of the antidiabetic potential of Zn-loaded Bryophyllum pinnatum (Lam.) extracts in experimental animal models. Futur J Pharm Sci 8, 42 (2022). https://doi.org/10.1186/s43094-022-00431-7
- Fasting blood glucose level